Chen, Yan, Ma, Jun, Yong, Yoong-Soon, Chen, Yonggan, Chen, Bing, Cao, Junming, Peng, Kai, Wang, Guaxia, Huang, Hai, and Loh, Jiun-Yan (2024) Impacts of Black Soldier Fly (Hermetia illucens) Larval Meal on Intestinal Histopathology and Microbiome Responses in Hybrid Grouper (Epinephelus fuscoguttatus ♀ × E. lanceolatus ♂): A Comprehensive Analysis. Animals, 14 (24). 3596.

Preview

PDF (Published Version) - Published Version Available under License Creative Commons Attribution. Download (5MB) | Preview

Abstract

This study examined the diversity and responses of intestinal microbiota in hybrid grouper (Epinephelus fuscoguttatus ♀ × E. lanceolatus ♂) fed diets with varying levels of fishmeal replaced by black soldier fly larvae (BSFL). The 10% BSFL substitution (BSFL10) group showed the highest levels of trypsin and amylase. Substituting fishmeal with 30% and 50% BSFL weakened the intestinal wall, resulting in vacuoles, sparse striatal boundaries, and fewer villi. Microbiota diversity, measured through Shannon’s index, was higher in the BSFL10 and BSFL50 groups than in the control. 16S rRNA amplicon data revealed the dominance of Firmicutes, Proteobacteria, Bacteroidetes, Spirochaetota, and Verrucomicrobia phyla. The BSFL-replacement groups showed an increase in Proteobacteria, Bacteroidetes, and Spirochaetota compared to the control, but fewer Firmicutes. PICRUSt analysis indicated significant alterations in microbial function, particularly enhanced protein, carbohydrate, lipid, and energy metabolisms in the BSFL-fed group. Substituting 10% fishmeal with BSFL enhanced nutrient metabolism and gut microbiota in juvenile hybrid grouper. Further research is needed to explore factors affecting the efficacy of insect feed as a sustainable aquaculture diet.

Item ID:	84339
Item Type:	Article (Research - C1)
ISSN:	2076-2615
Copyright Information:	© 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
Date Deposited:	16 Dec 2024 23:09
FoR Codes:	30 AGRICULTURAL, VETERINARY AND FOOD SCIENCES > 3005 Fisheries sciences > 300501 Aquaculture @ 100%
SEO Codes:	10 ANIMAL PRODUCTION AND ANIMAL PRIMARY PRODUCTS > 1002 Fisheries - aquaculture > 100299 Fisheries - aquaculture not elsewhere classified @ 100%
Downloads:	Total: 2 Last 12 Months: 2
	More Statistics