Chew, Xian Zhe, and Gibson-Kueh, Susan (2023) The haematology of clinically healthy, farmed juvenile Asian seabass (Lates calcarifer Bloch)-reference intervals, and indicators of subclinical disease. Journal of Fish Diseases. (In Press)

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Abstract

This study establishes the blood reference intervals (RIs) for clinically healthy and farmed juvenile Asian seabass (Lates calcarifer), within 4-6 weeks after stocking into flow-through, marine aquaculture systems. The 90% percentile RIs (n = 156, mean bodyweight 41.8 g) are as follows: glucose (GLU) 2.4-11.3 mmol/L, haematocrit (Hct) 18.9%-39.2%, haemoglobin concentration (Hb) 56.0-85.0 g/L, total plasma protein (TPP) 56.0-77.0 g/L, total red blood cell (RBC) count 4.1-11.2 × 1012 /L, total white blood cell (WBC) count 5.3-69.9 × 109 /L, total lymphocytes 4.7-51.4 × 109 /L, monocytes 0.3-16.2 × 109 /L and heterophils count 0.6-8.4 × 109 /L. Pearson's method analysis showed weak but significantly positive correlations between fish bodyweight and Hct, Hb, TPP and total RBC count (p < 0.05). Histopathology of 42 of the 156 clinically healthy fish used to derive the RIs, with blood values within the 90% percentile range, did not exhibit any abnormal pathology. In contrast, histopathology from a different group of clinically healthy L. calcarifer (n = 72, mean bodyweight 31.3 g) with blood values falling outside of these established 90% percentile RIs showed that 25% of these fish had severe, chronic granulomatous enteritis, and 13% had severely depleted lipid stores in their liver. Point biserial correlation analysis of blood values from this second group of 72 fish showed that elevated total WBC, monocyte and heterophil counts and reduced Hct levels are significantly associated (p < 0.05) with the occurrence of severe, chronic granulomatous enteritis and depleted lipid stores in their liver. Reduced blood GLU and TPP levels in the second group of fish were significantly associated with fish that had depleted lipid stores in liver (p < 0.05), corroborating a period of malnutrition. This study is among the first to establish blood RIs for clinically healthy, farmed juvenile L. calcarifer and detection of subclinical diseases in fish to support early intervention.

Item ID:	79975
Item Type:	Article (Research - C1)
ISSN:	1365-2761
Copyright Information:	This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. © 2023 The Authors. Journal of Fish Diseases published by John Wiley & Sons Ltd
Date Deposited:	28 Aug 2023 23:34
FoR Codes:	30 AGRICULTURAL, VETERINARY AND FOOD SCIENCES > 3099 Other agricultural, veterinary and food sciences > 309999 Other agricultural, veterinary and food sciences not elsewhere classified @ 50% 30 AGRICULTURAL, VETERINARY AND FOOD SCIENCES > 3005 Fisheries sciences > 300501 Aquaculture @ 50%
SEO Codes:	10 ANIMAL PRODUCTION AND ANIMAL PRIMARY PRODUCTS > 1002 Fisheries - aquaculture > 100202 Aquaculture fin fish (excl. tuna) @ 100%
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