Resolving the global distribution of sawfishes (family Pristidae) using environmental DNA

Cooper, Madalyn K. (2022) Resolving the global distribution of sawfishes (family Pristidae) using environmental DNA. PhD thesis, James Cook University.

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The imperiled status of sawfishes globally has warranted the development of robust and reliable tools to study them. Madalyn Cooper developed a non-invasive detection method harnessing environmental DNA and applied the method in a survey of coastal and riverine habitats in the global tropics and subtropics where the sawfishes face risk of extinction. The methods and findings of Madalyn's PhD are significant for agencies and practitioners working toward the management and conservation of sawfish populations, and furthers the application of eDNA methods for rare and threatened species.

Item ID: 77805
Item Type: Thesis (PhD)
Keywords: biodiversity assessment, conservation genetics, ecology of eDNA, eDNA sampling methods, eDNA, elasmobranch eDNA, environmental DNA, marine species monitoring, metabarcoding, method optimization, methodological considerations, non-invasive surveys, Pristidae, qPCR, sampling design, sawfishes, species distribution, threatened species, tropics
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Copyright Information: Copyright © 2022 Madalyn K. Cooper.
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Four publications arising from this thesis are stored in ResearchOnline@JCU, at the time of processing. Please see the Related URLs. The publications are:

[Chapter 2] Huerlimann, R., Cooper, M.K., Edmunds, R.C., Villacorta-Rath, C., Le Port, A., Robson, H.L.A., Strugnell, J.M., Burrows, D., and Jerry, D.R. (2020) Enhancing tropical conservation and ecology research with aquatic environmental DNA methods: an introduction for non-environmental DNA specialists. Animal Conservation, 23 (6). pp. 632-645.

[Chapter 2] Le Port, Agnes, Bakker, Judith, Cooper, Madalyn K., Huerlimann, Roger, and Mariani, Stefano (2018) Environmental DNA (eDNA): a valuable tool for ecological inference and management of sharks and their relatives. In: Carrier, Jeffrey C., Heithaus, Michael R., and Simpfendorfer, Colin A., (eds.) Shark Research: emerging technologies and applications for the field and laboratory. Marine Biology Series . CRC Press, Boca Raton, FL, USA, pp. 255-283.

[Chapter 3] Cooper, Madalyn K., Huerlimann, Roger, Edmunds, Richard C., Budd, Alyssa M., Le Port, Agnes, Kyne, Peter M., Jerry, Dean R., and Simpfendorfer, Colin A. (2021) Improved detection sensitivity using an optimal eDNA preservation and extraction workflow and its application to threatened sawfishes. Aquatic Conservation: marine and freshwater ecosystems, 31 (8). pp. 2131-2148.

[Chapter 4] Cooper, Madalyn K., Villacorta-Rath, Cecilia, Burrows, Damien, Jerry, Dean R., Carr, Leah, Barnett, Adam, Huveneers, Charlie, and Simpfendorfer, Colin A. (2022) Practical eDNA sampling methods inferred from particle size distribution and comparison of capture techniques for a Critically Endangered elasmobranch. Environmental DNA, 4 (5). pp. 1011-1023.

Date Deposited: 27 Feb 2023 02:46
FoR Codes: 31 BIOLOGICAL SCIENCES > 3199 Other biological sciences > 319902 Global change biology @ 30%
31 BIOLOGICAL SCIENCES > 3103 Ecology > 310305 Marine and estuarine ecology (incl. marine ichthyology) @ 35%
31 BIOLOGICAL SCIENCES > 3105 Genetics > 310599 Genetics not elsewhere classified @ 35%
SEO Codes: 18 ENVIRONMENTAL MANAGEMENT > 1805 Marine systems and management > 180504 Marine biodiversity @ 35%
18 ENVIRONMENTAL MANAGEMENT > 1802 Coastal and estuarine systems and management > 180299 Coastal and estuarine systems and management not elsewhere classified @ 35%
28 EXPANDING KNOWLEDGE > 2801 Expanding knowledge > 280102 Expanding knowledge in the biological sciences @ 30%
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