Impact of geography and time on genetic clusters of Opisthorchis viverrini identified by microsatellite and mitochondrial DNA analysis

Namsanor, Jutamas, Pitaksakulrat, Opal, Kopolrat, Kulthilda, Kiatsopit, Nadda, Webster, Bonnie L., Gower, C.M., Webster, Joanne P., Laha, Thewarach, Saijuntha, Weerachai, Laoprom, Nongluk, Andrews, Ross H., Petney, Trevor N., Blair, David, and Sithithaworn, Paiboon (2020) Impact of geography and time on genetic clusters of Opisthorchis viverrini identified by microsatellite and mitochondrial DNA analysis. International Journal for Parasitology, 50 (14). pp. 1133-1144.

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Abstract

Infection by the small liver fluke, Opisthorchis viverrini, causes serious public health problems, including cholangiocarcinoma, in Thailand and southeastern Asian countries. Previous studies have reported that O. viverrini represents a species complex with varying levels of genetic differentiation in Thailand and Lao PDR. In this study, we re-examined population genetic structure and genetic diversity of O. viverrini using extensive samples of the parasite collected over 15 years from 12 geographical localities in Thailand and eight localities in Lao PDR. Parasite life-cycle stages of 721 individuals of O. viverrini (91 cercariae, 230 metacercariae and 400 adult worms) were genotyped using 12 microsatellite loci. Metacercariae exhibited genetic diversity comparable with that of experimentally raised adults: metacercariae can therefore be used to represent O. viverrini populations without the need for laboratory definitive hosts. Data obtained from larval as well as adult worms identified two distinct genetic clusters of O. viverrini. Sequences of a portion of the mitochondrial cox1 gene strongly supported the existence of these two clusters. One, the widespread cluster, was found at all sampled sites. The second cluster occurred only in Phang Khon District, Sakon Nakhon Province (SPk), within the Songkram River wetland in Thailand. A striking feature of our data relates to the temporal dynamics of the SPk cluster, which was largely replaced by representatives of the widespread cluster over time. If the SPk cluster is excluded, no marked genetic differences were seen among O. viverrini populations from Thailand and Lao PDR. The underlying causes of the observed population structure and population dynamics of O. viverrini are not known.

Item ID: 65427
Item Type: Article (Research - C1)
ISSN: 1879-0135
Keywords: Opisthorchis viverrini; Population structure; Mitochondrial cox1 sequences; Microsatellites; Thailand; Lao PDR
Copyright Information: (C) 2020 Published by Elsevier Ltd on behalf of Australian Society for Parasitology. All rights reserved.
Funders: Khon Kaen University (KKU)
Projects and Grants: KKU Institute of Cholangiocarcinoma Research, KKU Invitation Research Fund (IN62138), KKU Faculty of Medicine
Date Deposited: 13 Jan 2021 22:39
FoR Codes: 31 BIOLOGICAL SCIENCES > 3105 Genetics > 310599 Genetics not elsewhere classified @ 50%
32 BIOMEDICAL AND CLINICAL SCIENCES > 3207 Medical microbiology > 320704 Medical parasitology @ 50%
SEO Codes: 92 HEALTH > 9204 Public Health (excl. Specific Population Health) > 920406 Food Safety @ 25%
92 HEALTH > 9204 Public Health (excl. Specific Population Health) > 920405 Environmental Health @ 25%
97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 50%
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