Molecular techniques and their limitations shape our view of the holobiont

Cooke, Ira, Mead, Oliver, Whalen, Casey, Boote, Chloe, Moya, Aurelie, Ying, Hua, Robbins, Steven, Strugnell, Jan M., Darling, Aaron, Miller, David, Voolstra, Christian, and Adamska, Maya (2019) Molecular techniques and their limitations shape our view of the holobiont. Zoology, 137. 125695.

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It is now recognised that the biology of almost any organism cannot be fully understood without recognising theexistence and potential functional importance of associated microbes. Arguably, the emergence of this holisticviewpoint may never have occurred without the development of a crucial molecular technique, 16S rDNAamplicon sequencing, which allowed microbial communities to be easily profiled across a broad range of con-texts. A diverse array of molecular techniques are now used to profile microbial communities, infer their evo-lutionary histories, visualise them in host tissues, and measure their molecular activity. In this review, we ex-amine each of these categories of measurement and inference with a focus on the questions they make tractable,and the degree to which their capabilities and limitations shape our view of the holobiont.

Item ID: 61455
Item Type: Article (Research - C1)
ISSN: 1873-2720
Keywords: Holobiont, Imaging, Metagenomic sequencing, Evolutionary inference, Model system, Multi-omics
Copyright Information: © 2019 Elsevier GmbH. All rights reserved.
Date Deposited: 10 Feb 2020 02:06
FoR Codes: 31 BIOLOGICAL SCIENCES > 3107 Microbiology > 310703 Microbial ecology @ 40%
31 BIOLOGICAL SCIENCES > 3101 Biochemistry and cell biology > 310199 Biochemistry and cell biology not elsewhere classified @ 20%
31 BIOLOGICAL SCIENCES > 3105 Genetics > 310509 Genomics @ 40%
SEO Codes: 97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 100%
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