Parasitic protozoan interactions with bacterial microbiome in a tropical fish farm

Gomes, Giana Bastos, Hutson, Kate S., Domingos, Jose A., Infante Villamil, Sandra, Huerlimann, Roger, Miller, Terrence L., and Jerry, Dean (2019) Parasitic protozoan interactions with bacterial microbiome in a tropical fish farm. Aquaculture, 502. pp. 196-201.

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Abstract

The bacterial microbiome is an important component of any aquaculture environment. The interaction between the bacterial microbiome and other microorganisms (e.g. parasites, viruses, or other bacteria) in aquaculture systems can prevent or contribute to disease outbreaks. This study characterised the bacterial composition associated with the abundance of a ciliated protozoan parasite, Chilodonella hexasticha, in gills and freshwater ponds of barramundi, Lates calcarifer, farm in tropical Queensland, Australia, over one year. An environmental DNA (eDNA) approach was used to estimate the abundance of C. hexasticha (copies/μl) in water through SSUrDNA gene qPCR and the relative abundance of bacterial species in water and fish gills through 16S rRNA V3 and V4 metabarcoding. The overall bacterial community diversity, dominated by Actinobacteria (42%), Proteobacteria (28%), Bacteroidetes (10%) and Cyanobacteria (6%), was stable among ponds over the study period (p > .05). Of those that could be identified to species, Flavobacterium columnare, Veillonella dispar and Bdellovibrio bacteriovorus abundance correlated with both high C. hexasticha levels in pond water and high observed fish mortalities (p < .05). Results also revealed significantly higher levels of F. columnare, B. bacteriovorus,Plesiomonas shigelloides, Prostecobactor debontii and Oxalobacter formigenes (p < .05) in gills of fish with high infection levels of C. hexasticha compared to fish with no detected parasite infection. This study demonstrated, for the first time, a link between increased parasitic ciliate abundance, bacterial composition and fish mortalities in a freshwater aquaculture environment and the application of eDNA to investigate pathogen, host and environment interactions.

Item ID: 56665
Item Type: Article (Refereed Research - C1)
Keywords: aquaculture; Chilodonella spp.; Ciliate protozoan; environmental DNA; Flavobacterium columnare; metabarcoding; microbiome
ISSN: 1873-5622
Funders: Queensland Government Smart Futures (QGSF), Fisheries Research and Development Corporation, James Cook University
Projects and Grants: QGSF Integrated management of parasite infections in tropical aquaculture
Date Deposited: 08 Jan 2019 00:58
FoR Codes: 07 AGRICULTURAL AND VETERINARY SCIENCES > 0704 Fisheries Sciences > 070401 Aquaculture @ 30%
07 AGRICULTURAL AND VETERINARY SCIENCES > 0704 Fisheries Sciences > 070404 Fish Pests and Diseases @ 70%
SEO Codes: 83 ANIMAL PRODUCTION AND ANIMAL PRIMARY PRODUCTS > 8301 Fisheries - Aquaculture > 830102 Aquaculture Fin Fish (excl. Tuna) @ 100%
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