Therapeutic trials against pre-existing Chequa iflavirus in redclaw crayfish (Cherax quadricarinatus)

Sakuna, Kitikarn, Elliman, Jennifer, and Owens, Leigh (2018) Therapeutic trials against pre-existing Chequa iflavirus in redclaw crayfish (Cherax quadricarinatus). Aquaculture, 492. pp. 9-14.

[img] PDF (Published Version) - Published Version
Restricted to Repository staff only

View at Publisher Website: http://dx.doi.org/10.1016/j.aquaculture....
 
1
1


Abstract

Chequa iflavirus is associated with mortalites reaching 20-40% after about three weeks following a stress event in farmed redclaw crayfish (Cherax quadricarinatus). Farmers need cost-effective interventions that can reduce the impact of this virus. This study was to identify strategies that would reduce the viral copy number as measured by reverse transcriptase quantitative polymerase chain reaction (RT-qPCR). With increasing viral copy number, there was significantly (P < 0.002) less weight gain at similar to 16% reduction in growth. Chequa iflavirus (> 10(4) copies) was distributed widely in all crayfish tissues sampled suggesting dispersion throughout the body via the haemolymph. In two experiments each of 70 crayfish in 2 x 5 treatments, the source of the crayfish, the polyphenol flavonoid quercetin, inhabiting water temperature at 32 degrees C all had statistically significant effects (P < 0.001; P < 0.018; P < 0.05 respectively). Viral copy numbers dropped 10-fold to 10(4.1) with quercetin and 82% to 10(2.75) with 32 degrees C water temperature. Unexpectedly, delivery of specific RNA interference (RNAi) within a plasmid allowed viral copy number to initially increase due to presumed activation of the inflammasome via the dsDNA plasmid (unmethylated CpG) or endotoxin (lipopolysaccharide) inclusion and immunosuppression. Nevertheless, RNAi followed its predicted half-life and by the end of the experiment, a 5-fold reduction of viral copy numbers had occurred. A one minute, 5000 ppm formalin dip of eggs dropped viral copy numbers by 99.997% to 2.4 x 10(3). Five crayfish out of 140 that were repetitively bled and tested 5 or 6 times were test-negative with RT-qPCR that had a sensitivity of 100 copies. They were likely to be virus-free and could be used to start a specific pathogen free breeding program. This study demonstrated a number of ways forward for the crayfish farmers with the inflammasome suppressant, quercetin being the most favoured as it is abundant, cheap and readily incorporated into the diet and should ameliorate the original, transport-stress mortality which started this study.

Item ID: 54341
Item Type: Article (Research - C1)
ISSN: 1873-5622
Keywords: Chequa iflavirus, stress, quercetin, temperature, Cherax quadricarinatus, RNAi
Funders: JCU, North Queensland Crayfish Farmers, Australian government - RIRDC
Projects and Grants: Rural Industries Research Development Corporation, Australia (PRJ-009388) initiated by the North Queensland Crayfish Farmers., JCU Collaborating Research Grant (2113.93474.2090) with the North Queensland Crayfish Farmers
Date Deposited: 27 Jun 2018 07:31
FoR Codes: 06 BIOLOGICAL SCIENCES > 0605 Microbiology > 060506 Virology @ 60%
07 AGRICULTURAL AND VETERINARY SCIENCES > 0704 Fisheries Sciences > 070401 Aquaculture @ 40%
SEO Codes: 83 ANIMAL PRODUCTION AND ANIMAL PRIMARY PRODUCTS > 8301 Fisheries - Aquaculture > 830101 Aquaculture Crustaceans (excl. Rock Lobster and Prawns) @ 80%
97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 20%
Downloads: Total: 1
More Statistics

Actions (Repository Staff Only)

Item Control Page Item Control Page