A novel virus (order Bunyavirales) from stressed redclaw crayfish (Cherax quadricarinatus) from farms in northern Australia

Sakuna, Kitikarn, Elliman, Jennifer, Tzamouzaki, Anna, and Owens, Leigh (2018) A novel virus (order Bunyavirales) from stressed redclaw crayfish (Cherax quadricarinatus) from farms in northern Australia. Virus Research, 250. pp. 7-12.

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Abstract

Athtabvirus, a bunya-like virus and chequa iflavirus infect redclaw crayfish (Cherax quadricarinatus) and they may cause mortality reaching 20-40% after about three weeks following transportation stress. Lesions were seen in the muscles of broodstock and juveniles and nerve cords of craylings. Using NextGen sequencing, the whole transcriptomes of a farmed case crayfish and a tank-reared, unaffected crayfish were assembled producing over 500,000 contigs. The average depth of reads was 18 replicates with a range from 15 to 44. The near complete sequences of the large and middle genome segments of a bunya-like virus were detected along with chequa iflavirus. The internal bunya-like motifs; RNA-dependent RNA polymerase on the L segment, and glycoprotein n (Gn) on the M segment were easily identified. In the opposite, positive-sense direction on the M segment, another presumed glycoprotein (glycoprotein c) with a low-density lipoprotein receptor (cysteine-rich) motif was identified by position specific iterated (psi)-BLASTp. The athtabvirus was related to Whenzhou Shrimp Virus 2 (E = 0.0, 43% amino acid identity), an unassigned, - ve sense ssRNA virus, and to peribunyaviruses (E = 10(-50-20)) In descending order of the number of RNA copies/0.2 mg of tissue, the organs most heavily infected were muscle (9.4 x 10(6)), nerve cord (5.24 x 10(6)), heart (4.07 x 10(6)), gills (3.96 x 10(6)), hepatopancreas (1.58 x 10(6)) and antennal gland (6.6 x 10(5)). Given the tissue tropism (muscle and nerves) of athtabvirus and the original lesions, this virus is implicated in being involved in the mortalities in crayfish after transportation.

Item ID: 54010
Item Type: Article (Research - C1)
ISSN: 1872-7492
Keywords: bunya-like virus, redclaw crayfish, Cherax quadricarinatus, stressed, Bunyavirales
Copyright Information: © 2018 Elsevier B.V. All rights reserved.
Funders: Australian Government, North Queensland Crayfish Farmers, JCU
Projects and Grants: JCU Collaborating Research Grant with the North Queensland Crayfish Farmers, grant from the Rural Industries Research Development Corporation, Australia to the North Queensland Crayfish Farmers
Date Deposited: 13 Jun 2018 07:30
FoR Codes: 06 BIOLOGICAL SCIENCES > 0605 Microbiology > 060506 Virology @ 60%
07 AGRICULTURAL AND VETERINARY SCIENCES > 0704 Fisheries Sciences > 070401 Aquaculture @ 40%
SEO Codes: 83 ANIMAL PRODUCTION AND ANIMAL PRIMARY PRODUCTS > 8301 Fisheries - Aquaculture > 830101 Aquaculture Crustaceans (excl. Rock Lobster and Prawns) @ 80%
97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 20%
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