Chemical imaging of a Symbiodinium sp. cell using synchrotron infrared microspectroscopy: a feasibility study

Gordon, B.R., Martin, D.E., Bambery, K.R., and Motti, C.A. (2018) Chemical imaging of a Symbiodinium sp. cell using synchrotron infrared microspectroscopy: a feasibility study. Journal of Microscopy, 270 (1). pp. 83-91.

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Abstract

The symbiotic relationship between corals and Symbiodinium spp. is the key to the success and survival of coral reef ecosystems the world over. Nutrient exchange and chemical communication between the two partners provides the foundation of this key relationship, yet we are far from a complete understanding of these processes. This is due, in part, to the difficulties associated with studying an intracellular symbiosis at the small spatial scales required to elucidate metabolic interactions between the two partners. This feasibility study, which accompanied a more extensive investigation of fixed Symbiodinium cells (data unpublished), examines the potential of using synchrotron radiation infrared microspectroscopy (SR-IRM) for exploring metabolite localisation within a single Symbiodinium cell. In doing so, three chemically distinct subcellular regions of a single Symbiodinium cell were established and correlated to cellular function based on assignment of diagnostic chemical classes.

Item ID: 53538
Item Type: Article (Research - C1)
ISSN: 1365-2818
Keywords: FTIR microspectroscopy, symbiodinium, synchrotron
Funders: Australian Synchrotron
Date Deposited: 09 May 2018 07:55
FoR Codes: 06 BIOLOGICAL SCIENCES > 0601 Biochemistry and Cell Biology > 060199 Biochemistry and Cell Biology not elsewhere classified @ 50%
06 BIOLOGICAL SCIENCES > 0601 Biochemistry and Cell Biology > 060101 Analytical Biochemistry @ 50%
SEO Codes: 97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 50%
96 ENVIRONMENT > 9608 Flora, Fauna and Biodiversity > 960808 Marine Flora, Fauna and Biodiversity @ 50%
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