Molecular changes in skin pigmented lesions of the coral trout Plectropomus leopardus

Lerebours, Adélaïde, Chapman, Emma C., Sweet, Michael J., Heupel, Michelle R., and Rotchell, Jeanette M. (2016) Molecular changes in skin pigmented lesions of the coral trout Plectropomus leopardus. Marine Environmental Research, 120. pp. 130-135.

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Abstract

A high prevalence of skin pigmented lesions of 15% was recently reported in coral trout Plectropomus leopardus, a commercially important marine fish, inhabiting the Great Barrier Reef. Herein, fish were sampled at two offshore sites, characterised by high and low lesion prevalence. A transcriptomic approach using the suppressive subtractive hybridisation (SSH) method was used to analyse the differentially expressed genes between lesion and normal skin samples. Transcriptional changes of 14 genes were observed in lesion samples relative to normal skin samples. These targeted genes encoded for specific proteins which are involved in general cell function but also in different stages disrupted during the tumourigenesis process of other organisms, such as cell cycling, cell proliferation, skeletal organisation and cell migration. The results highlight transcripts that are associated with the lesion occurrence, contributing to a better understanding of the molecular aetiology of this coral trout skin disease.

Item ID: 46395
Item Type: Article (Research - C1)
ISSN: 1879-0291
Keywords: fish, skin lesion, melanosis, gene expression
Funders: Fisheries Society of the British Isles (FSBI), Australian Research Council (ARC), Australian Institute of Marine Science (AIMS)
Projects and Grants: FSBI small research grant 2013, ARC Future Fellowship FT100101004
Date Deposited: 09 Nov 2016 07:38
FoR Codes: 06 BIOLOGICAL SCIENCES > 0604 Genetics > 060407 Genome Structure and Regulation @ 80%
06 BIOLOGICAL SCIENCES > 0602 Ecology > 060205 Marine and Estuarine Ecology (incl Marine Ichthyology) @ 20%
SEO Codes: 97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 100%
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