Iron acquisition in the marine actinomycete genus Salinispora is controlled by the desferrioxamine family of siderophores

Roberts, Alexandra A., Schultz, Andrew W., Kersten, Roland D., Dorrestein, Pieter C., and Moore, Bradley S. (2012) Iron acquisition in the marine actinomycete genus Salinispora is controlled by the desferrioxamine family of siderophores. FEMS Microbiology Letters, 335 (2). pp. 95-103.

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Abstract

Many bacteria produce siderophores for sequestration of growth-essential iron. Analysis of the Salinispora genomes suggests that these marine actinomycetes support multiple hydroxamate- and phenolate-type siderophore pathways. We isolated and characterized desferrioxamines (DFOs) B and E from all three recognized Salinispora species and linked their biosyntheses in S. tropica CNB-440 and S. arenicola CNS-205 to the des locus through PCR-directed mutagenesis. Gene inactivation of the predicted iron-chelator biosynthetic loci sid2-4 did not abolish siderophore chemistry. Additionally, these pathways could not restore the native growth characteristics of the des mutants in iron-limited media, although differential iron-dependent regulation was observed for the yersiniabactin-like sid2 pathway. Consequently, this study indicates that DFOs are the primary siderophores in laboratory cultures of Salinispora.

Item ID: 25716
Item Type: Article (Research - C1)
ISSN: 1574-6968
Keywords: genome mining; pathway mutagenesis; siderophore chemistry
Date Deposited: 14 May 2013 01:21
FoR Codes: 06 BIOLOGICAL SCIENCES > 0604 Genetics > 060405 Gene Expression (incl Microarray and other genome-wide approaches) @ 10%
06 BIOLOGICAL SCIENCES > 0605 Microbiology > 060504 Microbial Ecology @ 50%
06 BIOLOGICAL SCIENCES > 0605 Microbiology > 060503 Microbial Genetics @ 40%
SEO Codes: 97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 100%
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