The neuronal calcium sensor protein acrocalcin: a potential target of calmodulin regulation during development in the coral Acropora millepora

Reyes-Bermudez, Alejandro, Miller, David J., and Sprungala, Susanne (2012) The neuronal calcium sensor protein acrocalcin: a potential target of calmodulin regulation during development in the coral Acropora millepora. PLoS ONE, 7 (12). 0051689. pp. 1-8.

[img] PDF (Published Version) - Published Version
Available under License Creative Commons Attribution.

Download (4MB)
View at Publisher Website: http://dx.doi.org/10.1371/journal.pone.0...
 
8
82


Abstract

To understand the calcium-mediated signalling pathways underlying settlement and metamorphosis in the Scleractinian coral Acropora millepora, a predicted protein set derived from larval cDNAs was scanned for the presence of EF-hand domains (Pfam Id: PF00036). This approach led to the identification of a canonical calmodulin (AmCaM) protein and an uncharacterised member of the Neuronal Calcium Sensor (NCS) family of proteins known here as Acrocalcin (AmAC). While AmCaM transcripts were present throughout development, AmAC transcripts were not detected prior to gastrulation, after which relatively constant mRNA levels were detected until metamorphosis and settlement. The AmAC protein contains an internal CaM-binding site and was shown to interact in vitro with AmCaM. These results are consistent with the idea that AmAC is a target of AmCaM in vivo, suggesting that this interaction may regulate calcium-dependent processes during the development of Acropora millepora.

Item ID: 25073
Item Type: Article (Research - C1)
ISSN: 1932-6203
Funders: ARC Centre of Excellence for Coral Reef Studies, James Cook University
Date Deposited: 20 Feb 2013 09:07
FoR Codes: 06 BIOLOGICAL SCIENCES > 0604 Genetics > 060405 Gene Expression (incl Microarray and other genome-wide approaches) @ 60%
06 BIOLOGICAL SCIENCES > 0603 Evolutionary Biology > 060309 Phylogeny and Comparative Analysis @ 20%
03 CHEMICAL SCIENCES > 0301 Analytical Chemistry > 030104 Immunological and Bioassay Methods @ 20%
SEO Codes: 97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 100%
Downloads: Total: 82
Last 12 Months: 1
More Statistics

Actions (Repository Staff Only)

Item Control Page Item Control Page