Seddon, J.M. (2005) Canid-specific primers for molecular sexing using tissue or non-invasive samples. Conservation Genetics, 6 (1). pp. 147-149.

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Abstract

[Extract] There is an increasing use of non-invasive samples for studying wild animal populations both to avoid disturbance of the animals and to facilitate the collection of samples. An important component for conservation studies is the accurate identification of the sex of individuals. However, some sexing methods encounter difficulties in the amplification of long fragments from degraded DNAandinnon-specific amplification from faeces analysis in carnivores. Here I present a method for sexing of wolves by a multiplex PCR amplification that is particularly suited to non-invasive samples. This method can easily be adapted for a wide range of species.

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