Protocol for identification of proteins from deyolked zebrafish embryos
Purushothaman, Kathiresan, Tneo Rwei Qing, Saraphina Dianne, Ee, Yong Xin, Ananthakrishnan, Nithiyakala, Harshiktha, Shanmugam, Vij, Shubha, Lin, Qingsong, and Babiak, Igor (2025) Protocol for identification of proteins from deyolked zebrafish embryos. STAR Protocols, 6 (2). 103728.
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Abstract
Zebrafish is a key model for studying vertebrate development and human diseases, but proteomic data during embryogenesis are limited due to interference from egg yolk proteins. Here, we present a protocol for the isolation, identification, and analysis of proteins from zebrafish embryos. We describe steps for dechorionation, deyolking, protein extraction, SDS-PAGE, and trypsin digestion. We then detail procedures for peptide separation using liquid chromatography-tandem mass spectrometry (LC-MS/MS), identification via ProteinPilot, and functional analysis with Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology. For complete details on the use and execution of this protocol, please refer to Purushothaman et al.<sup>1</sup>
| Item ID: | 88482 |
|---|---|
| Item Type: | Article (Scholarly Work) |
| ISSN: | 2666-1667 |
| Keywords: | developmental biology, molecular biology, protein expression and purification |
| Copyright Information: | © 2025 The Author(s). Attribution-NonCommercial-NoDerivatives 4.0 International. |
| Date Deposited: | 29 Apr 2026 01:49 |
| FoR Codes: | 30 AGRICULTURAL, VETERINARY AND FOOD SCIENCES > 3005 Fisheries sciences > 300501 Aquaculture @ 100% |
| SEO Codes: | 10 ANIMAL PRODUCTION AND ANIMAL PRIMARY PRODUCTS > 1002 Fisheries - aquaculture > 100202 Aquaculture fin fish (excl. tuna) @ 100% |
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