Protocol for feeding strategy and proteomics analysis of zebrafish Danio rerio using S-trap and iTRAQ techniques
Purushothaman, Kathiresan, Kaul, Daanish, Rwei Qing, Saraphina Dianne Tneo, Rocha, Sérgio D.C., Göksu, Aleksandar B., Morales Lange, Byron, Mydland, Liv Torunn, Vij, Shubha, Qingsong, Lin, Øverland, Margareth, and Press, Charles Mc L. (2024) Protocol for feeding strategy and proteomics analysis of zebrafish Danio rerio using S-trap and iTRAQ techniques. STAR Protocols, 5 (4). 103513.
|
PDF (Published Version)
- Published Version
Available under License Creative Commons Attribution Non-commercial No Derivatives. Download (3MB) | Preview |
Abstract
Yeast serves as a functional alternative and sustainable protein source in aquaculture. This protocol outlines feeding strategy and intestinal proteome analysis of zebrafish (Danio rerio), using S-trap for digestion, iTRAQ, and mass spectrometry for protein quantification. Additionally, it details the analysis of chemical components in feed and functional assessments via Kyoto Encyclopedia of Genes and Genomes (KEGG), Eukaryotic Orthologous Group (KOG), and Gene Ontology (GO). For complete details on the use and execution of this protocol, please refer to Purushothaman et al.<sup>1</sup>
| Item ID: | 86902 |
|---|---|
| Item Type: | Article (Scholarly Work) |
| ISSN: | 2666-1667 |
| Keywords: | immunology, model organisms, proteomics |
| Copyright Information: | © 2024 The Author(s). Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
| Date Deposited: | 06 Nov 2025 03:24 |
| FoR Codes: | 30 AGRICULTURAL, VETERINARY AND FOOD SCIENCES > 3005 Fisheries sciences > 300501 Aquaculture @ 100% |
| SEO Codes: | 10 ANIMAL PRODUCTION AND ANIMAL PRIMARY PRODUCTS > 1002 Fisheries - aquaculture > 100202 Aquaculture fin fish (excl. tuna) @ 100% |
| Downloads: |
Total: 1 Last 12 Months: 1 |
| More Statistics |
Actions (Repository Staff Only)
 |
Item Control Page |