Purificacion, Marynold, Shah, Roslina Binti Mohd, De Meeûs, Thierry, Bakar, Saripah Binti, Savantil, Anisah Bintil, Yusof, Meriam Mohd, Amalin, Divina, Nguyen, Hien, Sulistyowati, Endang, Budiman, Aris, Ekayanti, Arni, Niogret, Jerome, Ravel, Sophie, Vreysen, Marc J B., and Abd-Alla, Adly M.M. (2024) Development and characterization of microsatellite markers for population genetics of the cocoa pod borer Conopomorpha cramerella (Snellen) (Lepidoptera: Gracillaridae). PLoS ONE, 19 (4). e0297662.

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Abstract

The cocoa pod borer (CPB) Conopomorpha cramerella (Snellen) (Lepidoptera: Gracillaridae) is one of the major constraints for cocoa production in South East Asia. In addition to cultural and chemical control methods, autocidal control tactics such as the Sterile Insect Technique (SIT) could be an efficient addition to the currently control strategy, however SIT implementation will depend on the population genetics of the targeted pest. The aim of the present work was to search for suitable microsatellite loci in the genome of CPB that is partially sequenced. Twelve microsatellites were initially selected and used to analyze moths collected from Indonesia, Malaysia, and the Philippines. A quality control verification process was carried out and seven microsatellites found to be suitable and efficient to distinguish differences between CPB populations from different locations. The selected microsatellites were also tested against a closely related species, i.e. the lychee fruit borer Conopomorpha sinensis (LFB) from Vietnam and eight loci were found to be suitable. The availability of these novel microsatellite loci will provide useful tools for the analysis of the population genetics and gene flow of these pests, to select suitable CPB strains to implement the SIT.

Item ID:	85964
Item Type:	Article (Research - C1)
ISSN:	1932-6203
Copyright Information:	© 2024 Purificacion et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Date Deposited:	25 Jun 2025 00:08
FoR Codes:	30 AGRICULTURAL, VETERINARY AND FOOD SCIENCES > 3008 Horticultural production > 300804 Horticultural crop protection (incl. pests, diseases and weeds) @ 50% 31 BIOLOGICAL SCIENCES > 3105 Genetics > 310509 Genomics @ 50%
SEO Codes:	26 PLANT PRODUCTION AND PLANT PRIMARY PRODUCTS > 2605 Horticultural crops > 260599 Horticultural crops not elsewhere classified @ 100%
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