Dong, Ruiqi, Qiu, Jianqiang, Cao, Junming, Huang, Wen, Chen, Bing, Zhao, Hongxia, Sun, Wenhao, Lu, Huijie, Loh, Jiun-Yan, and Peng, Kai (2025) In vitro and in vivo evaluation of the effects of condensed tannins and catechins monomers on antioxidant and intestinal health of Chinese seabass (Lateolabrax maculatus). Frontiers in Veterinary Science, 12. 1558942.

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Abstract

Plant-derived condensed tannins (CT) exhibit strong bioactivity of antioxidant, immunostimulation and intestinal protection, but with little clues of the mechanism of action. Since CT are consist of catechins (CAs) monomers, e.g., catechin (CA), epicatechin (EC) and epigallocatechin (EG), we motivated to use the monomers to explore the underlying mechanisms in a seabass model focusing on anti-oxidative stress and intestinal health of Lateolabrax maculatus. An in vitro intestinal primary cell oxidative stress model induced by hydrogen peroxide was set up to assess the antioxidant and immune activities of CT and CAs. Another 56–d feeding trial with 800 fish was conducted to evaluate the effects of CT and CAs on growth performance, intestinal permeability and digestive enzyme activities, intestinal morphology and antioxidant status, and intestinal bacterial flora of fish. Five diets were prepared to contain 0 (G1) and 1 g/kg of CT, CA, EC and EG. Fish were randomly distributed into 20 tanks with 4 tanks per diet and 40 fish per tank, and were fed to apparent satiation twice daily. Results showed that CT and CAs exhibited similar effects in alleviating hydrogen peroxide-induced cell injury by activating nuclear factor erythroid 2-related factor 2 gene expression, and improving antioxidant and immune capacities. Dietary CT and CAs enhanced intestinal antioxidant ability and increased (p < 0.05) the abundance of intestinal Firmicutes, Proteobacteria and Bacteroidetes to oxidative stress tolerant. With a dose of 1 g/kg CT and CA promoted (p < 0.05) intestinal total antioxidant capacity, but slightly induced intestinal injury mainly due to increased (p < 0.05) intestinal permeability (as reflected by increased lipopolysaccharide concentrations) and inhibited (p < 0.05) digestion (as reflected by the decreased trypsin and lipase activities) of fish. In summary, CT and CAs protect intestine from oxidative stress and improve intestinal antioxidant capacity by stimulating antioxidant enzyme system and bacterial flora. CA and EC show similar or superior antioxidant activity than CT.

Item ID:	84777
Item Type:	Article (Research - C1)
ISSN:	2297-1769
Copyright Information:	Copyright © 2025 Dong, Qiu, Cao, Huang, Chen, Zhao, Sun, Lu, Loh and Peng. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Date Deposited:	28 Feb 2025 00:58
FoR Codes:	30 AGRICULTURAL, VETERINARY AND FOOD SCIENCES > 3005 Fisheries sciences > 300501 Aquaculture @ 100%
SEO Codes:	10 ANIMAL PRODUCTION AND ANIMAL PRIMARY PRODUCTS > 1002 Fisheries - aquaculture > 100202 Aquaculture fin fish (excl. tuna) @ 100%
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