Identification, synthesis, conformation and activity of an insulin‐like peptide from a sea anemone

Mitchell, Michela L., Hossain, Mohammed Akhter, Lin, Feng, Pinheiro‐Junior, Ernesto L., Peigneur, Steve, Wai, Dorothy C.C., Delaine, Carlie, Blyth, Andrew J., Forbes, Briony E., Tytgat, Jan, Wade, John D., and Norton, Raymond S. (2021) Identification, synthesis, conformation and activity of an insulin‐like peptide from a sea anemone. Biomolecules, 11 (12). 1785.

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The role of insulin and insulin‐like peptides (ILPs) in vertebrate animals is well studied. Numerous ILPs are also found in invertebrates, although there is uncertainty as to the function and role of many of these peptides. We have identified transcripts with similarity to the insulin family in the tentacle transcriptomes of the sea anemone Oulactis sp. (Actiniaria: Actiniidae). The translated transcripts showed that these insulin‐like peptides have highly conserved A‐ and B‐chains among individuals of this species, as well as other Anthozoa. An Oulactis sp. ILP sequence (IlO1_i1) was synthesized using Fmoc solid‐phase peptide synthesis of the individual chains, followed by regi-oselective disulfide bond formation of the intra‐A and two interchain disulfide bonds. Bioactivity studies of IlO1_i1 were conducted on human insulin and insulin‐like growth factor receptors, and on voltage‐gated potassium, sodium, and calcium channels. IlO1_i1 did not bind to the insulin or insulin‐like growth factor receptors, but showed weak activity against KV1.2, 1.3, 3.1, and 11.1 (hERG) channels, as well as NaV1.4 channels. Further functional studies are required to determine the role of this peptide in the sea anemone.

Item ID: 82700
Item Type: Article (Research - C1)
ISSN: 2218-273X
Keywords: Cnidaria, Insulin, Invertebrates, Ion channel, Oulactis, Peptide synthesis
Copyright Information: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (
Funders: Australian Research Council (ARC)
Projects and Grants: ARC LP150100621, ARC APP1117483
Date Deposited: 08 May 2024 23:49
FoR Codes: 31 BIOLOGICAL SCIENCES > 3109 Zoology > 310902 Animal cell and molecular biology @ 50%
31 BIOLOGICAL SCIENCES > 3103 Ecology > 310305 Marine and estuarine ecology (incl. marine ichthyology) @ 50%
SEO Codes: 28 EXPANDING KNOWLEDGE > 2801 Expanding knowledge > 280102 Expanding knowledge in the biological sciences @ 100%
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