Structural insights into the assembly and regulation of distinct viral capsid complexes

Sarker, Subir, Terrón, María C., Khandokar, Yogesh, Aragão, David, Hardy, Joshua M., Radjainia, Mazdak, Jiménez-Zaragoza, Manuel, De Pablo, Pedro J., Coulibaly, Fasséli, Luque, Daniel, Raidal, Shane R., and Forwood, Jade K. (2016) Structural insights into the assembly and regulation of distinct viral capsid complexes. Nature Communications, 7. 13014.

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Abstract

The assembly and regulation of viral capsid proteins into highly ordered macromolecular complexes is essential for viral replication. Here, we utilize crystal structures of the capsid protein from the smallest and simplest known viruses capable of autonomously replicating in animal cells, circoviruses, to establish structural and mechanistic insights into capsid morphogenesis and regulation. The beak and feather disease virus, like many circoviruses, encode only two genes: a capsid protein and a replication initiation protein. The capsid protein forms distinct macromolecular assemblies during replication and here we elucidate these structures at high resolution, showing that these complexes reverse the exposure of the N-terminal arginine rich domain responsible for DNA binding and nuclear localization. We show that assembly of these complexes is regulated by single-stranded DNA (ssDNA), and provide a structural basis of capsid assembly around single-stranded DNA, highlighting novel binding interfaces distinct from the highly positively charged N-terminal ARM domain.

Item ID: 79858
Item Type: Article (Research - C1)
ISSN: 2041-1723
Copyright Information: © The Author(s) 2016. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
Funders: Australian Research Council (ARC)
Date Deposited: 30 Aug 2023 07:11
FoR Codes: 31 BIOLOGICAL SCIENCES > 3107 Microbiology > 310706 Virology @ 100%
SEO Codes: 28 EXPANDING KNOWLEDGE > 2801 Expanding knowledge > 280102 Expanding knowledge in the biological sciences @ 100%
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