Genome sequence of an Australian strain of canid alphaherpesvirus 1

Sarker, S., Das, S., Helbig, K., Peters, A., and Raidal, S.R. (2018) Genome sequence of an Australian strain of canid alphaherpesvirus 1. Australian Veterinary Journal, 96 (1-2). pp. 24-27.

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Objective: Characterisation of a complete genome sequence of an Australian strain of canid alphaherpesvirus 1 (CHV-1) and its phylogenetic relationship with other varicellovirus species.

Methods: Standard pathology and PCR methods were used to initially detect herpesvirus in hepatic tissue from an infected 4-week-old Labrador Retriever puppy. The complete CHV-1 genome was sequenced using next-generation sequencing technology followed by de novo and reference assembly, and genome annotation.

Results: The CHV-1 genome was 125 kbp in length and contained 74 predicted open reading frames encoding functional proteins, all of which have counterparts in other alphaherpesviruses. Phylogenetic analysis using the DNA polymerase gene revealed that the newly sequenced CHV-1 clustered with canid alphaherpesvirus isolated from the UK and shared a 99% overall nucleotide sequence similarity.

Conclusion: This is the first complete genome of an Australian strain of CHV-1, which will contribute to our understanding of the genetics and evolution of herpesvirus.

Item ID: 79849
Item Type: Article (Short Note)
ISSN: 1751-0813
Keywords: acute hepatitis, dogs, herpesvirus, next-generation sequencing, whole genome
Copyright Information: © 2017 Australian Veterinary Association.
Date Deposited: 13 Sep 2023 07:02
FoR Codes: 31 BIOLOGICAL SCIENCES > 3105 Genetics > 310509 Genomics @ 50%
30 AGRICULTURAL, VETERINARY AND FOOD SCIENCES > 3009 Veterinary sciences > 300914 Veterinary virology @ 50%
SEO Codes: 28 EXPANDING KNOWLEDGE > 2801 Expanding knowledge > 280101 Expanding knowledge in the agricultural, food and veterinary sciences @ 50%
28 EXPANDING KNOWLEDGE > 2801 Expanding knowledge > 280102 Expanding knowledge in the biological sciences @ 50%
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