High-Density Genetic Linkage Map of the Southern Blue-ringed Octopus (Octopodidae: Hapalochlaena maculosa)

Whitelaw, Brooke L., Jones, David B., Guppy, Jarrod, Morse, Peter, Strugnell, Jan M., Cooke, Ira R., and Zenger, Kyall (2022) High-Density Genetic Linkage Map of the Southern Blue-ringed Octopus (Octopodidae: Hapalochlaena maculosa). Diversity, 14 (12). 1068.

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Abstract

Genetic linkage maps provide a useful resource for non-model genomes and can aid in genome reassembly to form more contiguous pseudo-chromosomes. We present the first linkage map of any cephalopod, H. maculosa, composed of 47 linkage groups (LG). A total of 2166 single nucleotide polymorphisms and 2455 presence–absence variant loci were utilised by Lep-Map3 in linkage map construction. The map length spans 2016.62 cM with an average marker distance of 0.85 cM. Integration of the recent H. maculosa genome allowed 1151 scaffolds comprising 34% of the total genomic sequence to be orientated and/or placed using 1278 markers across all 47 LG. The linkage map generated provides a new perspective on HOX gene distribution in octopods. In the H. maculosa linkage map three (SCR, LOX4 and POST1) of six identified HOX genes (HOX1/LAB, SCR, LOX2, LOX4, LOX5, POST1) were located within the same LG (LG 9). The generation of a linkage map for H. maculosa has provided a valuable resource for understanding the evolution of cephalopod genomes and will provide a base for future work.

Item ID: 77548
Item Type: Article (Research - C1)
ISSN: 1424-2818
Keywords: cephalopods, enetic linkage, genome evolution, Hapalochlaena, HOX genes
Copyright Information: © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
Date Deposited: 16 Feb 2023 00:44
FoR Codes: 31 BIOLOGICAL SCIENCES > 3104 Evolutionary biology > 310403 Biological adaptation @ 20%
31 BIOLOGICAL SCIENCES > 3105 Genetics > 310506 Gene mapping @ 30%
31 BIOLOGICAL SCIENCES > 3105 Genetics > 310508 Genome structure and regulation @ 50%
SEO Codes: 18 ENVIRONMENTAL MANAGEMENT > 1899 Other environmental management > 189999 Other environmental management not elsewhere classified @ 20%
28 EXPANDING KNOWLEDGE > 2801 Expanding knowledge > 280102 Expanding knowledge in the biological sciences @ 80%
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