Identification of vicilin, legumin and antimicrobial peptide 2a as macadamia nut allergens
Kabasser, Stefan, Pratap, Kunal, Kamath, Sandip, Taki, Aya, Dang, Thanh, Koplin, Jennifer, Perrett, Kirsten, Hummel, Karin, Radauer, Christian, Breiteneder, Heimo, Lopata, Andreas L., and Bublin, Merima (2022) Identification of vicilin, legumin and antimicrobial peptide 2a as macadamia nut allergens. Food Chemistry, 370. 131028.
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Abstract
Macadamia nut is an increasingly popular food item of a healthy diet. However, macadamia nut is also a potent allergenic food. To date, there is little information about the allergenic proteins involved. In this study, using sera from macadamia nut allergic individuals, four IgE-binding proteins were detected. Their identities were determined by tandem mass spectrometry with de novo sequencing. Three IgE-reactive proteins, the vicilin Mac i 1, the legumin Mac i 2 and the antimicrobial peptide 2a/Mac i 1 (28–76) were purified from the nut while the nonspecific lipid transfer protein was produced as a recombinant in Pichia pastoris. IgE-binding assays using sera from well-characterized groups of tree nut and/or peanut allergic patients revealed that the allergens were mainly recognized by sera from macadamia nut allergic individuals. Hence, these newly discovered allergens will enable molecular diagnostics to identify patients at high risk of macadamia nut allergy.
| Item ID: | 69562 |
|---|---|
| Item Type: | Article (Research - C1) |
| ISSN: | 1873-7072 |
| Keywords: | Allergen, Food allergens, Food allergy, Legumin, Macadamia allergy, Macadamia nut, Vicilin, Tree nut allergy |
| Copyright Information: | © 2021 Elsevier Ltd. All rights reserved. |
| Funders: | National Health and Medical Research Council (NHMRC) |
| Projects and Grants: | NHMRC GNT1124143 |
| Date Deposited: | 06 Oct 2021 00:33 |
| FoR Codes: | 32 BIOMEDICAL AND CLINICAL SCIENCES > 3204 Immunology > 320401 Allergy @ 33% 31 BIOLOGICAL SCIENCES > 3101 Biochemistry and cell biology > 310101 Analytical biochemistry @ 33% 31 BIOLOGICAL SCIENCES > 3101 Biochemistry and cell biology > 310109 Proteomics and intermolecular interactions (excl. medical proteomics) @ 34% |
| SEO Codes: | 20 HEALTH > 2004 Public health (excl. specific population health) > 200405 Food safety @ 50% 28 EXPANDING KNOWLEDGE > 2801 Expanding knowledge > 280103 Expanding knowledge in the biomedical and clinical sciences @ 50% |
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