PCR, in-situ hybridization, and phylogenetic analysis suggest that ‘big belly’ disease in barramundi, Lates calcarifer (Bloch), is caused by a novel Vibrio species

Gibson-Kueh, Susan, Terence, Celestine, Chew, Xian Zhe, Uichanco, Joseph Angelo, and Shen, Xueyan (2021) PCR, in-situ hybridization, and phylogenetic analysis suggest that ‘big belly’ disease in barramundi, Lates calcarifer (Bloch), is caused by a novel Vibrio species. Journal of Fish Diseases. (In Press)

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Abstract

‘Big belly’ disease is a chronic, granulomatous bacterial enteritis and peritonitis, first reported in 3- to 4-week-old Asian seabass or barramundi, Lates calcarifer Bloch fry. Affected fry are emaciated and have a swollen abdomen, and the condition is referred to as ‘skinny pot-belly’ or ‘big belly’ disease. In this study, histopathological examinations of diseased fish from a batch of 2-month-old, 6- to 8-cm L. calcarifer fingerlings, kept in seawater recirculating aquaculture systems, showed pathology resembling ‘big belly’ disease. Ethanol-fixed tissues were tested positive using specific PCR primers based on 16S rRNA genes. In situ hybridization using dioxygenin-labelled positive PCR products on formalin-fixed paraffin-embedded tissues showed positive reactions with intralesional, clusters of the large, ‘big belly’ coccobacilli. A phylogenetic tree constructed based on analyses of these 16S rRNA gene PCR products from five positive fish suggests that the ‘big belly’ bacterium is most likely a novel Vibrio species.

Item ID: 69094
Item Type: Article (Research - C1)
ISSN: 1365-2761
Keywords: aquaculture, Asian seabass, barramundi, Edwardsiella spp., fish disease, Vibrio spp.
Copyright Information: © 2021 John Wiley & Sons Ltd
Date Deposited: 22 Aug 2021 23:43
FoR Codes: 31 BIOLOGICAL SCIENCES > 3109 Zoology > 310905 Animal immunology @ 100%
SEO Codes: 10 ANIMAL PRODUCTION AND ANIMAL PRIMARY PRODUCTS > 1002 Fisheries - aquaculture > 100202 Aquaculture fin fish (excl. tuna) @ 100%
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