Commercial fish ELISA kits have a limited capacity to detect different fish species and their products

Ruethers, Thimo, Taki, Aya C., Khangurha, Jasmit, Roberts, James, Buddhadasa, Saman, Clarke, Dean, Hedges, Claire E., Campbell, Dianne E., Kamath, Sandip D., Lopata, Andreas L., and Koeberl, Martina (2020) Commercial fish ELISA kits have a limited capacity to detect different fish species and their products. Journal of the Science of Food and Agriculture, 100 (12). pp. 4353-4363.

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Abstract

BACKGROUND: Fish is a major food and allergen source, requiring safety declarations on packages. Enzyme-linked immunosorbent assays (ELISAs) are often used to ensure that the product meets the required standards with regard to the presence of allergens. Over 1000 different fish species are traded and consumed worldwide, and they are increasingly provided by aquaculture. Up to 3% of the general population is at risk of sometimes fatal allergic reactions to fish, requiring strict avoidance of this commodity. The aim of this study is to evaluate the capacity of three commercially available ELISA tests to detect a wide variety bony and cartilaginous fish and their products, which is essential to ensure reliable and safe food labeling.

RESULTS: The detection rates for 57 bony fish ranged from 26% to 61%. Common European and North American species, including carp, cod, and salmon species, demonstrated a higher detection rate than those from the Asia-Pacific region, including pangasius, and several mackerel and tuna species. Among the 17 canned bony fish products, only 65% to 86% were detected, with tuna showing the lowest rate. None of the cartilaginous fish (n = 9), other vertebrates (n = 8), or shellfish (n = 5) were detected.

CONCLUSIONS: We demonstrated that three commercial fish ELISA kits had a limited capacity to detect fish and their products. The complexity of fish as a protein source that is increasingly utilized means that there is an urgent need for improved detection methods. This is crucial for the food industry to provide safe seafood products and comply with international legislation.

Item ID: 66243
Item Type: Article (Research - C1)
ISSN: 1097-0010
Keywords: bony fish; cartilaginous fish; fish allergy; fish cross-reactivity; food labeling; food safety
Copyright Information: © 2020 Society of Chemical Industry
Funders: National Health and Medical Research Council of Australia (NHMRC)
Projects and Grants: NHMRC APP1086656
Date Deposited: 16 Mar 2021 02:30
FoR Codes: 30 AGRICULTURAL, VETERINARY AND FOOD SCIENCES > 3006 Food sciences > 300605 Food safety, traceability, certification and authenticity @ 50%
32 BIOMEDICAL AND CLINICAL SCIENCES > 3204 Immunology > 320401 Allergy @ 50%
SEO Codes: 20 HEALTH > 2004 Public health (excl. specific population health) > 200405 Food safety @ 50%
28 EXPANDING KNOWLEDGE > 2801 Expanding knowledge > 280101 Expanding knowledge in the agricultural, food and veterinary sciences @ 50%
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