Expanding the allergen repertoire of salmon and catfish

Ruethers, Thimo, Taki, Aya C., Karnaneedi, Shaymaviswanathan, Nie, Shuai, Kalic, Tanja, Dai, Danyi, Daduang, Sakda, Leeming, Michael, Williamson, Nicholas A., Breiteneder, Heimo, Mehr, Sam S., Kamath, Sandip, Campbell, Dianne E., and Lopata, Andreas (2020) Expanding the allergen repertoire of salmon and catfish. Allergy, 76 (5). pp. 1443-1453.

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Abstract

Background: Diagnostic tests for fish allergy are hampered by the large number of under‐investigated fish species. Four salmon allergens are well‐characterized and registered with the WHO/IUIS while no catfish allergens have been described so far. In 2008, freshwater‐cultured catfish production surpassed that of salmon, the globally most‐cultured marine species. We aimed to identify, quantify, and compare all IgE‐binding proteins in salmon and catfish.

Methods: Seventy‐seven pediatric patients with clinically confirmed fish allergy underwent skin prick tests to salmon and catfish. The allergen repertoire of raw and heated protein extracts was evaluated by immunoblotting using five allergen‐specific antibodies and patients' serum followed by mass spectrometric analyses.

Results: Raw and heated extracts from catfish displayed a higher frequency of IgE‐binding compared to those from salmon (77% vs 70% and 64% vs 53%, respectively). The major fish allergen parvalbumin demonstrated the highest IgE‐binding capacity (10%‐49%), followed by triosephosphate isomerase (TPI; 19%‐34%) in raw and tropomyosin (6%‐32%) in heated extracts. Six previously unidentified fish allergens, including TPI, were registered with the WHO/IUIS. Creatine kinase from salmon and catfish was detected by IgE from 14% and 10% of patients, respectively. Catfish L‐lactate dehydrogenase, glyceraldehyde‐3‐phosphate dehydrogenase, pyruvate kinase, and glucose‐6‐phosphate isomerase showed IgE‐binding for 6%‐13% of patients. In salmon, these proteins could not be separated successfully.

Conclusions: We detail the allergen repertoire of two highly farmed fish species. IgE‐binding to fish tropomyosins and TPIs was demonstrated for the first time in a large patient cohort. Tropomyosins, in addition to parvalbumins, should be considered for urgently needed improved fish allergy diagnostics.

Item ID: 66238
Item Type: Article (Research - C1)
ISSN: 1398-9995
Keywords: fish allergy diagnosis, Pangasianodon hypophthalmus, Salmo salar, triosephosphate isomerase, tropomyosin
Copyright Information: © 2020 EAACI and John Wiley and Sons A/S. Published by John Wiley and Sons Ltd.
Funders: James Cook University, Centre for Food and Allergy Research, National Health and Medical Research Council (NHMRC), Australian Research Council (ARC), Khon Kaen University (KKU)
Projects and Grants: NHMRC grant APP1086656, NHMRC grant GNT1124143, ARC Peter Doherty Early Career Research Fellow (GNT1124143), KKU Research and Technology Transfer Affairs
Date Deposited: 09 Mar 2021 23:57
FoR Codes: 30 AGRICULTURAL, VETERINARY AND FOOD SCIENCES > 3006 Food sciences > 300605 Food safety, traceability, certification and authenticity @ 50%
32 BIOMEDICAL AND CLINICAL SCIENCES > 3204 Immunology > 320401 Allergy @ 50%
SEO Codes: 20 HEALTH > 2004 Public health (excl. specific population health) > 200405 Food safety @ 50%
28 EXPANDING KNOWLEDGE > 2801 Expanding knowledge > 280101 Expanding knowledge in the agricultural, food and veterinary sciences @ 50%
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