Roles of gonad-inhibiting hormone in the protandric simultaneous hermaphrodite peppermint shrimp

Shi, Wenyuan, Liu, Fang, Liu, An, Huang, Huiyang, Lin, Qi, Zeng, Chaoshu, and Ye, Haihui (2020) Roles of gonad-inhibiting hormone in the protandric simultaneous hermaphrodite peppermint shrimp. Biology of Reproduction, 103 (4). pp. 817-827.

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Abstract

To date, the molecular mechanisms of the unique gonadal development mode known as protandric simultaneous hermaphroditism (PSH) are unclear in crustaceans. In this study, cDNA of a gonadinhibiting hormone (Lv-GIH1) was isolated from the PSH peppermint shrimp Lysmata vittata, and its expression was exclusively found in the eyestalk ganglion. Real-time quantitative polymerase chain reaction (qRT-PCR) revealed that the expression of Lv-GIH1 increased during gonadal development of the functional male stages but decreased significantly at subsequent simultaneous hermaphroditism stage. Further in vitro experiment showed that recombinant GIH1 protein (rGIH1) effectively inhibited Vg expression in the cultured hepatopancreas tissues while the short-term injection of G/H1-dsRNA resulted in reduced expression of Lv-GIH1 and upregulated expression of Vg in the hepatopancreas. Moreover, long-term rGIH1 injection led to significantly reduced expression of Lv-Vg, Lv-VgR, and Lv-CFSH1, subdued growth of oocytes, and feathery setae as a secondary sexual characteristic in females. Interestingly, while germ cells in testicular part were suppressed by rGIH1 injection, the expression of Lv-IAGs showed no significant difference; and long-term GIH1-dsRNA injection results were contrary to those of rGIH1 injection. Taken together, the results of this study indicate that Lv-GIH1 is involved in gonadal development and might also participate in controlling secondary sexual characteristic development in L. vittata by inhibiting Lv-CFSH1 expression.

Item ID: 66052
Item Type: Article (Research - C1)
ISSN: 1529-7268
Keywords: crustacean, gonad-inhibiting hormone, gonad, secondary sexual characteristic, protandric simultaneous hermaphroditism
Copyright Information: © The Author(s) 2020. Published by Oxford University Press on behalf of the American Physical Therapy Association.
Date Deposited: 06 Jan 2021 07:31
FoR Codes: 30 AGRICULTURAL, VETERINARY AND FOOD SCIENCES > 3005 Fisheries sciences > 300501 Aquaculture @ 40%
31 BIOLOGICAL SCIENCES > 3109 Zoology > 310903 Animal developmental and reproductive biology @ 30%
31 BIOLOGICAL SCIENCES > 3109 Zoology > 310913 Invertebrate biology @ 30%
SEO Codes: 10 ANIMAL PRODUCTION AND ANIMAL PRIMARY PRODUCTS > 1002 Fisheries - aquaculture > 100201 Aquaculture crustaceans (excl. rock lobster and prawns) @ 60%
28 EXPANDING KNOWLEDGE > 2801 Expanding knowledge > 280102 Expanding knowledge in the biological sciences @ 40%
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