What lies beneath: Hydra provides cnidarian perspectives into the evolution of FGFR docking proteins

Suryawanshi, Ashwini, Schaefer, Karolin, Holz, Oliver, Apel, David, Lange, Ellen, Hayward, David C., Miller, David J., and Hassel, Monika (2020) What lies beneath: Hydra provides cnidarian perspectives into the evolution of FGFR docking proteins. Development Genes and Evolution, 230. pp. 227-238.

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Abstract

Across the Bilateria, FGF/FGFR signaling is critical for normal development, and in both Drosophila and vertebrates, docking proteins are required to connect activated FGFRs with downstream pathways. While vertebrates use Frs2 to dock FGFR to the RAS/MAPK or PI3K pathways, the unrelated protein, downstream of FGFR (Dof/stumps/heartbroken), fulfills the corresponding function in Drosophila. To better understand the evolution of the signaling pathway downstream of FGFR, the available sequence databases were screened to identify Frs2, Dof, and other key pathway components in phyla that diverged early in animal evolution. While Frs2 homologues were detected only in members of the Bilateria, canonical Dof sequences (containing Dof, ankyrin, and SH2/SH3 domains) were present in cnidarians as well as bilaterians (but not in other animals or holozoans), correlating with the appearance of FGFR. Although these data suggested that Dof coupling might be ancestral, gene expression analysis in the cnidarian Hydra revealed that Dof is not upregulated in the zone of strong FGFRa and FGFRb expression at the bud base, where FGFR signaling controls detachment. In contrast, transcripts encoding other, known elements of FGFR signaling in Bilateria, namely the FGFR adaptors Grb2 and Crkl, which are acting downstream of Dof (and Frs2), as well as the guanyl nucleotide exchange factor Sos, and the tyrosine phosphatase Csw/Shp2, were strongly upregulated at the bud base. Our expression analysis, thus, identified transcriptional upregulation of known elements of FGFR signaling at the Hydra bud base indicating a highly conserved toolkit. Lack of transcriptional Dof upregulation raises the interesting question, whether Hydra FGFR signaling requires either of the docking proteins known from Bilateria.

Item ID: 62704
Item Type: Article (Research - C1)
ISSN: 1432-041X
Keywords: Receptor tyrosine kinase, Adapter protein, Grb2, Crkl, Dof
Copyright Information: This article is licensed under a Creative CommonsAttribution 4.0 International License, which permits use, sharing,adaptation, distribution and reproduction in any medium or format, aslong as you give appropriate credit to the original author(s) and thesource, provide a link to the Creative Commons licence, and indicate ifchanges were made. The images or other third party material in this articleare included in the article's Creative Commons licence, unless indicatedotherwise in a credit line to the material. If material is not included in thearticle's Creative Commons licence and your intended use is notpermitted by statutory regulation or exceeds the permitted use, you willneed to obtain permission directly from the copyright holder. To view acopy of this licence, visithttp://creativecommons.org/licenses/by/4.0/.
Funders: Deutsche Forschungsgemeinschaft (DFG)
Projects and Grants: DFG grant HA1732/12, DFG grant HA1732/13
Research Data: david.miller@jcu.edu.au
Date Deposited: 08 Apr 2020 07:35
FoR Codes: 31 BIOLOGICAL SCIENCES > 3105 Genetics > 310510 Molecular evolution @ 100%
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