Genetic parameters for tolerance to gill-associated virus under challenge-test conditions in the black tiger shrimp (Penaeus monodon)

Noble, Tansyn N., Coman, Gregory J., Wade, Nicholas M., Thomson, Peter C., Raadsma, Herman W., Khatkar, Mehar S., Guppy, Jarrod L., and Jerry, Dean R. (2020) Genetic parameters for tolerance to gill-associated virus under challenge-test conditions in the black tiger shrimp (Penaeus monodon). Aquaculture, 516. 734428.

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Abstract

Diseases in shrimp aquaculture cause significant economic losses, particularly those caused by viruses. Genetic improvement of disease tolerance through selective breeding can be an important tool for managing the risk of disease in shrimp farming as long as the trait has a genetic basis (i.e. heritable) and is able to be accurately and reliably measured. This study presents the first estimates of genetic variation associated with tolerance to gill-associated virus (GAV) in Penaeus monodon using a unique controlled challenge test design. Shrimp families (n = 72) were communally spawned and reared prior to replicated communal challenge testing when they reached an average size of ~8 g. Pedigree determination was carried out using genotyping and shrimp were challenged by intramuscular injection with a weight standardised dose of GAV. Daily mortality data were collected over 15 days from 1717 individuals and GAV infection load was measured on the challenge test survivors (n = 963). Overall mortality after 15 days was 35.5% and ranged from 0 to 71% among families. Heritability estimates for mortality were 0.11 ± 0.03 using a binomial model and 0.14 (standard error not estimated) using Cox's proportional hazards model. The estimated heritability for GAV infection load was 0.21 ± 0.07. The genetic correlation between mortality and GAV infection load was not significantly different from zero, rg = 0.30 ± 0.23, suggesting that the two traits may be genetically independent, at least in the way they were measured in this study. Results from this study suggest a low but significant genetic basis for GAV tolerance in P. monodon and that selection may be a viable option to manage GAV-related disease. The study also demonstrated, for the first time in shrimp, communal-based spawning and rearing can be used for large-scale genetic analysis of disease tolerance, eliminating the need for single family rearing tanks and associated confounding environmental effects.

Item ID: 61447
Item Type: Article (Research - C1)
ISSN: 1873-5622
Keywords: Disease tolerance, Heritability, Selective breeding, Gill-associated virus, Challenge test, Black tiger shrimp
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Copyright Information: © 2019 Elsevier B.V. All rights reserved.
Additional Information:

A version of this publication was included as Chapter 5 of the following PhD thesis: Noble, Tansyn Honi (2018) Selective breeding for tolerance to gill-associated virus in the black tiger shrimp, Penaeus monodon. PhD thesis, James Cook University, which is available Open Access in ResearchOnline@JCU. Please see the Related URLs for access.

Date Deposited: 11 Feb 2020 01:51
FoR Codes: 07 AGRICULTURAL AND VETERINARY SCIENCES > 0704 Fisheries Sciences > 070401 Aquaculture @ 33%
07 AGRICULTURAL AND VETERINARY SCIENCES > 0704 Fisheries Sciences > 070404 Fish Pests and Diseases @ 33%
07 AGRICULTURAL AND VETERINARY SCIENCES > 0704 Fisheries Sciences > 070405 Fish Physiology and Genetics @ 34%
SEO Codes: 83 ANIMAL PRODUCTION AND ANIMAL PRIMARY PRODUCTS > 8301 Fisheries - Aquaculture > 830101 Aquaculture Crustaceans (excl. Rock Lobster and Prawns) @ 100%
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