Cryoprotective effect of glycerol against sperm DNA damage in frozen-thawed boar spermatozoa.

Pena, Santiago, Gummow, Bruce, Parker, Anthony, and Paris, Damien (2019) Cryoprotective effect of glycerol against sperm DNA damage in frozen-thawed boar spermatozoa. In: [Presented at the 86th Philippine Veterinary Medical Association Scientific Conference]. From: 86th Philippine Veterinary Medical Association Scientific Conference and Annual Convention, 20-22 February 2019, Iloilo, Philippines.

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Abstract

The use of frozen-thawed boar sperm still lags behind chilled boar semen in artificial insemination (AI) operations despite its advantages [1]. Freezing however, may damage the structure and function of sperm, leading to reduced fertilization success. Moreover, glycerol, which is a critical cryoprotectant in most sperm freezing protocols, can be toxic to cells in high concentrations [2]; affecting sperm motility and acrosomal integrity. This study was conducted to determine the cryoprotective effect of glycerol on sperm DNA integrity and motility in frozen-thawed boar sperm.

Item ID: 57398
Item Type: Conference Item (Abstract / Summary)
Keywords: boar, pig, sperm, DNA damage, cryopreservation, freezing, glycerol, sperm motility
Funders: AusAID, James Cook University
Projects and Grants: Australian Awards Scholarship, JCU Development Grant
Date Deposited: 22 Jul 2019 00:49
FoR Codes: 07 AGRICULTURAL AND VETERINARY SCIENCES > 0702 Animal Production > 070206 Animal Reproduction @ 50%
06 BIOLOGICAL SCIENCES > 0601 Biochemistry and Cell Biology > 060103 Cell Development, Proliferation and Death @ 30%
10 TECHNOLOGY > 1001 Agricultural Biotechnology > 100199 Agricultural Biotechnology not elsewhere classified @ 20%
SEO Codes: 83 ANIMAL PRODUCTION AND ANIMAL PRIMARY PRODUCTS > 8303 Livestock Raising > 830308 Pigs @ 40%
97 EXPANDING KNOWLEDGE > 970107 Expanding Knowledge in the Agricultural and Veterinary Sciences @ 30%
97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 30%
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