Highly polymorphic microsatellite loci for the Acapulco damselfish, Stegastes acapulcoensis, and cross amplification in three congeneric species

Rodríguez-Moreno, Melina, Zapata, Fernando A., Ramírez-Calero, Sandra, van Herwerden, Lynne, and Feldheim, Kevin (2019) Highly polymorphic microsatellite loci for the Acapulco damselfish, Stegastes acapulcoensis, and cross amplification in three congeneric species. Marine Biodiversity, 49 (1). pp. 481-486.

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Abstract

In the present study we report a set of 13 novel microsatellites isolated from and characterized for the Acapulco damselfish Stegastes acapulcoensis, a species endemic to, but widely distributed within, the Eastern Tropical Pacific (ETP) region. The loci were tested in 30 individuals from the Colombian Pacific and were highly polymorphic. The mean allele number per locus was 19.5 (+/- 4.03 SD) and the observed heterozygosities ranged from 0.192 to 1. Some of the loci were successfully cross-amplified and were polymorphic in three Stegastes species from the ETP: S. arcifrons, S. flavilatus and S. beebei (with 11, three and seven amplified loci, respectively). The high variability and cross-amplification success of the new set of microsatellites reported here allows these markers to be a useful resource for genetic studies of S. acapulcoensis and some of their congeners to address evolutionary, ecological and conservation-related questions in the Eastern Tropical Pacific.

Item ID: 57338
Item Type: Article (Research - C1)
ISSN: 1867-1624
Keywords: Stegastes, marker isolation, microsatellites, cross-amplification, population genetics, Eastern Pacific endemics
Copyright Information: © Senckenberg Gesellschaft für Naturforschung and Springer-Verlag GmbH Germany 2017
Funders: Colombian Administrative Department of Science, Technology and Innovation (COLCIENCIAS)
Projects and Grants: COLCIENCIAS grant No. FP44842-540-2014
Date Deposited: 06 Mar 2019 07:48
FoR Codes: 05 ENVIRONMENTAL SCIENCES > 0502 Environmental Science and Management > 050202 Conservation and Biodiversity @ 50%
06 BIOLOGICAL SCIENCES > 0604 Genetics > 060411 Population, Ecological and Evolutionary Genetics @ 50%
SEO Codes: 96 ENVIRONMENT > 9608 Flora, Fauna and Biodiversity > 960808 Marine Flora, Fauna and Biodiversity @ 100%
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