Assessment of a cricket, Acheta domesticus, bioassay for Chequa Iflavirus and bunya-like virus from redclaw crayfish Cherax quadricarinatus

Sakuna, Kitikarn, Elliman, Jennifer, and Owens, Leigh (2017) Assessment of a cricket, Acheta domesticus, bioassay for Chequa Iflavirus and bunya-like virus from redclaw crayfish Cherax quadricarinatus. Journal of Invertebrate Pathology, 150. pp. 41-44.

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View at Publisher Website: http://doi.org/10.1016/j.jip.2017.09.005
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Abstract

Chequa iflavirus and a bunya-like virus infect redclaw crayfish (Cherax quadricarinatus) and they may cause mortality reaching 20–40% after about three weeks after a stress event. To complete River’s postulates for viruses, virus-free animals are needed. Due to a lack of chequa iflavirus and bunya-like virus-free crayfish (testing shows >85% infection rate) coupled with the facts that iflavirus and bunyaviruses are found in insects and that crickets had been successful alternate hosts for crustacean viruses before, Acheta domesticus was trialled as a bioassay animal. There was no significant difference (P > 0.05) in mortality rates between uninfected control crickets and infected crickets. Reverse transcriptase polymerase chain reaction for both viruses failed to find any trace of the RNA viruses in fed or inoculated crickets after 30 days. The search for an alternative bioassay host will have to be widened.

Item ID: 51039
Item Type: Article (Research - C1)
ISSN: 1096-0805
Keywords: Chequa iflavirus, redclaw cray fish, Cherax quadricarinatus, crickets, Bunya-like virus
Funders: Rajamangala University of Technology, Rural Industries Research Development Corporation, James Cook University, North Queensland Crayfish Farmers
Date Deposited: 05 Oct 2017 00:57
FoR Codes: 06 BIOLOGICAL SCIENCES > 0605 Microbiology > 060506 Virology @ 50%
07 AGRICULTURAL AND VETERINARY SCIENCES > 0704 Fisheries Sciences > 070404 Fish Pests and Diseases @ 50%
SEO Codes: 83 ANIMAL PRODUCTION AND ANIMAL PRIMARY PRODUCTS > 8301 Fisheries - Aquaculture > 830101 Aquaculture Crustaceans (excl. Rock Lobster and Prawns) @ 100%
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