Validation of a portable, waterproof blood pH analyser for elasmobranchs

Talwar, Brendan, Bouyoucos, Ian A., Shipley, Oliver, Rummer, Jodie L., Mandelman, John W., Brooks, Edward J., and Grubbs, R. Dean (2017) Validation of a portable, waterproof blood pH analyser for elasmobranchs. Conservation Physiology, 5 (1).

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Abstract

Quantifying changes in blood chemistry in elasmobranchs can provide insights into the physiological insults caused by anthropogenic stress, and can ultimately inform conservation and management strategies. Current methods for analysing elasmobranch blood chemistry in the field are often costly and logistically challenging. We compared blood pH values measured using a portable, waterproof pH meter (Hanna Instruments HI 99161) with blood pH values measured by an i- STAT system (CG4+ cartridges), which was previously validated for teleost and elasmobranch fishes, to gauge the accuracy of the pH meter in determining whole blood pH for the Cuban dogfish (Squalus cubensis) and lemon shark (Negaprion brevirostris). There was a significant linear relationship between values derived via the pH meter and the i- STAT for both species across a wide range of pH values and temperatures (Cuban dogfish: 6.8-7.1 pH 24-30 degrees C; lemon sharks: 7.0-7.45 pH 25-31 degrees C). The relative error in the pH meter's measurements was similar to +/- 2.7%. Using this device with appropriate correction factors and consideration of calibration temperatures can result in both a rapid and accurate assessment of whole blood pH, at least for the two elasmobranch species examined here. Additional species should be examined in the future across a wide range of temperatures to determine whether correction factors are universal.

Item ID: 50501
Item Type: Article (Research - C1)
ISSN: 2051-1434
Keywords: blood pH, i-STAT, Negaprion brevirostris, point-of-care device, Squalus cubensis, stress
Additional Information:

© The Author 2017. Published by Oxford University Press and the Society for Experimental Biology. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/ by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

Funders: PADI Foundation, Sigma Xi Society, Guy Harvey Ocean Foundation, New England Aquarium, Florida State Coastal & Marine Laboratory, Cape Eleuthera Founation
Date Deposited: 20 Sep 2017 09:40
FoR Codes: 06 BIOLOGICAL SCIENCES > 0606 Physiology > 060603 Animal Physiology Systems @ 50%
06 BIOLOGICAL SCIENCES > 0602 Ecology > 060205 Marine and Estuarine Ecology (incl Marine Ichthyology) @ 50%
SEO Codes: 97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 100%
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