Resilience to ocean acidification: decreased carbonic anhydrase activity in sea anemones under high pCO2 conditions

Ventura, Patricia, Jarrold, Michael D., Merle, Pierre Laurent, Barnay-Verdier, Stéphanie, Zamoum, Thamilla, Rodolfo-Metalpa, Riccardo, Calosi, Piero, and Furla, Paola (2016) Resilience to ocean acidification: decreased carbonic anhydrase activity in sea anemones under high pCO2 conditions. Marine Ecology Progress Series, 559. pp. 257-263.

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Abstract

Non-calcifying photosynthetic anthozoans have emerged as a group that may thrive under high carbon dioxide partial pressure ( pCO2) conditions via increased productivity. However, the physiological mechanisms underlying this potential success are unclear. Here we investigated the impact of high pCO2 on the dissolved inorganic carbon (DIC) use in the temperate sea anemone Anemonia viridis. We assessed the impacts of long-term exposure to high pCO2, i.e. sampling in situ natural CO2 vents (Vulcano, Italy), and short-term exposure, i.e. during a 3 wk controlled laboratory experiment. We focused on photo-physiological parameters (net photosynthesis rates, chlorophyll a content and Symbiodinium density) and on carbonic anhydrase (CA) activity, an enzyme involved in the energy-demanding process of DIC absorption. Long-term exposure to high pCO2 had no impact on Symbiodinium density and chlorophyll a content. In contrst, short-term exposure to high pCO2 induced a significant reduction in Symbiodinium density, which together with unchanged net photosynthesis resulted in the increase of Symbiodinium productivity per cell. Finally, in both in situ long-term and laboratory short-term exposure to high pCO2, we observed a significant decrease in the CA activity of sea anemones, suggesting a change in DIC use (i.e. from an HCO3- to a CO2 user). This change could enable a shift in the energy budget that may increase the ability of non-calcifying photosynthetic anthozoans to cope with ocean acidification.

Item ID: 49183
Item Type: Article (Research - C1)
ISSN: 1616-1599
Keywords: dissolved inorganic carbon uptake, carbonic anhydrase, ocean acidification, plasticity, CO2 vent, Anemonia viridis
Funders: Ministère de l’Enseignement supérieur et de la Rechercherieur et de la Recherche (MESR), NERC, NSERC
Projects and Grants: MESR 513- EDSFA021-2013, EUFP7 MedSeA project (grant agreement no. 265103), NERC OA Research Programme
Date Deposited: 13 Jun 2017 01:30
FoR Codes: 06 BIOLOGICAL SCIENCES > 0602 Ecology > 060205 Marine and Estuarine Ecology (incl Marine Ichthyology) @ 50%
06 BIOLOGICAL SCIENCES > 0601 Biochemistry and Cell Biology > 060101 Analytical Biochemistry @ 50%
SEO Codes: 96 ENVIRONMENT > 9611 Physical and Chemical Conditions of Water > 961104 Physical and Chemical Conditions of Water in Marine Environments @ 50%
96 ENVIRONMENT > 9608 Flora, Fauna and Biodiversity > 960808 Marine Flora, Fauna and Biodiversity @ 50%
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