Mosquito cells (C6/36) fail to support the complete replication of Penaeus merguiensis hepandensovirus

Syahidah, D., Elliman, J., Constantinoiu, C., and Owens, L. (2017) Mosquito cells (C6/36) fail to support the complete replication of Penaeus merguiensis hepandensovirus. Journal of Invertebrate Pathology, 145. pp. 31-38.

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Abstract

Mosquito cell lines (C6/36) were reported in the literature to support the propagation of Penaeus monodon hepandensovirus (PmoHDV). We aim to evaluate the susceptibility and viral propagation of P. merguiensis hepandensovirus (PmeHDV) which is ∼22% different to PmoHDV in Aedes albopictus cell line (C6/36). Cellular changes in the infected cell culture were detected. Vacuole formation was seen in both infected and uninfected cell cultures. The average number of disrupted cellular membranes in the infected cells (presumptive dead cells) was significantly higher than that of uninfected cells at passage two (F = 9.749, d.f. 1, 22, p < 0.05). Using a proliferation assay, light absorption of infected cells peaked at 2 weeks post-infection (O.D. = 0.27) but was significantly lower than that of the uninfected groups (O.D. = 0.37) (F = 6.879, d.f. 1, 94, p < 0.05) suggesting hindered cell growth. PCR of the serial passages of the infected cell cultures indicated weak positive results for PmeHDV infection and TaqMan quantitative PCR confirmed that the average number of viral copies declined from 3.8 × 105 to 5.69 × 102 copies per μL and the mean of cycle times increased from 19.26 to 27.63. These results are interpreted to mean C6/36 allows the initial stage of PmeHDV replication, but the virus was incapable of using C6/36 for patent replication of its’ virions.

Item ID: 48095
Item Type: Article (Research - C1)
ISSN: 1096-0805
Keywords: Mosquito cells; C6/36; Penaeus merguiensis hepandensovirus; TaqMan qPCR; HPV
Date Deposited: 29 Mar 2017 02:10
FoR Codes: 06 BIOLOGICAL SCIENCES > 0605 Microbiology > 060506 Virology @ 50%
07 AGRICULTURAL AND VETERINARY SCIENCES > 0704 Fisheries Sciences > 070401 Aquaculture @ 50%
SEO Codes: 83 ANIMAL PRODUCTION AND ANIMAL PRIMARY PRODUCTS > 8301 Fisheries - Aquaculture > 830105 Aquaculture Prawns @ 100%
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