A panel of microsatellite loci from two species of octopus, Pareledone turqueti (Joubin, 1905) and Pareledone charcoti (Joubin, 1905)

Strugnell, Jan M., Allcock, A. Louise, and Watts, Phillip C. (2009) A panel of microsatellite loci from two species of octopus, Pareledone turqueti (Joubin, 1905) and Pareledone charcoti (Joubin, 1905). Molecular Ecology Resources, 9 (4). pp. 1239-1242.

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Eighteen dinucleotide microsatellite loci were isolated from two octopus species, Pareledone turqueti and Pareledone charcoti, which are endemic to the Southern Ocean. Genetic diversity was assessed in samples of P. charcoti and P. turqueti from Elephant Island and Shag Rocks respectively. All except one locus (which has proved to be polymorphic in other species) were variable in the focal species and amplified between six and 30 and between four and 28 alleles for P. charcoti and P. turqueti respectively; mean expected heterozygosities varied between 0.38 and 0.95 (P. charcoti) and between 0.34 and 0.97 (P. turqueti), with significant (P < 0.05) departures from Hardy-Weinberg equilibrium at seven loci; three of these loci provided significant (P < 0.05) evidence for null alleles. Two pairs of loci isolated from P. turqueti demonstrated significant (P < 0.05) linkage disequilibrium. We are presently using these-genetic markers to quantify spatial genetic structure in the genus Pareledone.

Item ID: 46357
Item Type: Article (Research - C1)
ISSN: 1755-0998
Keywords: Antarctica, microsatellites, Pareledone charcoti, Pareledone turqueti, population structure, Southern Ocean
Funders: National Environmental Research Council (NERC), Lloyd’s Tercentenary Fellowship (LTF), Antarctic Science Bursary (ASB), Systematics Association, Edith Mary Pratt Musgrave Fund (EMPMF)
Projects and Grants: NERC AFI NE/C50632/1, CoSyst
Date Deposited: 16 Nov 2016 07:45
FoR Codes: 06 BIOLOGICAL SCIENCES > 0604 Genetics > 060411 Population, Ecological and Evolutionary Genetics @ 100%
SEO Codes: 96 ENVIRONMENT > 9608 Flora, Fauna and Biodiversity > 960808 Marine Flora, Fauna and Biodiversity @ 100%
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