Characterisation of eleven new polymorphic microsatellite markers for the coastal stingray Dasyatis brevicaudata (Dasyatidae Hutton 1875), and cross-amplification in seven dasyatid species

Le Port, A., Roycroft, E.J., Thakur, V., and Lavery, S.D. (2016) Characterisation of eleven new polymorphic microsatellite markers for the coastal stingray Dasyatis brevicaudata (Dasyatidae Hutton 1875), and cross-amplification in seven dasyatid species. Biochemical Systematics and Ecology, 65. pp. 234-237.

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Abstract

Twelve microsatellite loci were developed for the short-tailed stingray Dasyatis brevicaudata using a next-generation sequencing approach, and their utility for population genetics studies was assessed. Eleven out of twelve loci were polymorphic (4-15 alleles) and amplified reliably, with observed heterozygosities between 0.32 and 0.86 (mean = 0.56). Successful cross-species amplification of many of these markers was achieved across seven other dasyatid species currently listed as "Data Deficient" by the IUCN, suggesting these microsatellites will be a useful resource for the investigation of ecological and conservation genetics in a range of species of conservation concern.

Item ID: 45448
Item Type: Article (Research - C1)
ISSN: 1873-2925
Keywords: coastal elasmobranch, microsatellite, conservation, habitat degradation, population genetics, PCR primers
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Funders: University of Auckland (UA)
Projects and Grants: UA NZGL00225
Date Deposited: 22 Jun 2016 07:49
FoR Codes: 06 BIOLOGICAL SCIENCES > 0604 Genetics > 060411 Population, Ecological and Evolutionary Genetics @ 100%
SEO Codes: 97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 100%
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