Characterization and cross-amplification of microsatellite markers in four species of anemonefish (Pomacentridae, Amphiprion spp.)

Bonin, Mary C., Saenz-Agudelo, Pablo, Harrison, Hugo B., Nanninga, Gerrit B., van der Meer, Martin H., Mansour, Hicham, Perumal, Sadhavisan, Jones, Geoffrey P., and Berumen, Michael L. (2016) Characterization and cross-amplification of microsatellite markers in four species of anemonefish (Pomacentridae, Amphiprion spp.). Marine Biodiversity, 46 (1). pp. 135-140.

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Abstract

Anemonefish are iconic symbols of coral reefs and have become model systems for research on larval dispersal and population connectivity in coral reef fishes. Here we present 24 novel microsatellite markers across four species of anemonefish and also test 35 previously published markers for cross-amplification on two anemonefish species in order to facilitate further research on their population genetics and phylogenetics. Novel loci were isolated from sequences derived from microsatellite-enriched or 454 GS-FLX shotgun sequence libraries developed using congeneric DNA. Primer testing successfully identified 15 new microsatellite loci for A. percula, 4 for A. melanopus, 3 for A. akindynos, and 2 for A. omanensis. These novel microsatellite loci were polymorphic with a mean of 10 +/- 1.6 SE (standard error) alleles per locus and an average observed heterozygosity of 0.647 +/- 0.032 SE. Reliable cross-amplification of 12 and 26 of the 35 previously published Amphiprion markers was achieved for A. melanopus and A. akindynos, respectively, suggesting that the use of markers developed from the DNA of congeners can provide a quick and cost-effective alternative to the isolation of new loci. Together, the markers presented here provide an important resource for ecological, evolutionary, and conservation genetic research on anemonefishes that will inform broader conservation and management actions for coral reef fishes.

Item ID: 44058
Item Type: Article (Research - C1)
ISSN: 1867-1624
Keywords: Amphiprion, clownfish, coral reefs, microsatellite, population genetics
Funders: King Abdullah University of Science and Technology (KAUST), National Environmental Research Program (NERP) Tropical Ecosystems Hub, Australian Research Council Centre of Excellence for Coral Reef Studies, KAUST Red Sea Research Center, KAUST Biosciences Core Laboratory
Projects and Grants: KAUST Award CRG-1-2012-BER-002, KAUST Award OCRF-SPCF-2011-BER-001, NERP Project 8.3
Date Deposited: 27 Apr 2016 07:38
FoR Codes: 06 BIOLOGICAL SCIENCES > 0602 Ecology > 060205 Marine and Estuarine Ecology (incl Marine Ichthyology) @ 100%
SEO Codes: 96 ENVIRONMENT > 9608 Flora, Fauna and Biodiversity > 960808 Marine Flora, Fauna and Biodiversity @ 100%
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