Transcriptome analysis in Haematococcus pluvialis: astaxanthin induction by Salicylic Acid (SA) and Jasmonic Acid (JA)

Gao, Zhengquan, Li, Yan, Wu, Guanxun, Li, Guoqiang, Sun, Haifeng, Deng, Suzhen, Shen, Yicheng, Chen, Guoqiang, Zhang, Ruihao, Meng, Chunxiao, and Zhang, Xiaowen (2015) Transcriptome analysis in Haematococcus pluvialis: astaxanthin induction by Salicylic Acid (SA) and Jasmonic Acid (JA). PLoS ONE, 10 (10). pp. 1-14.

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Abstract

Haematococcus pluvialis is an astaxanthin-rich microalga that can increase its astaxanthin production by salicylic acid (SA) or jasmonic acid (JA) induction. The genetic transcriptome details of astaxanthin biosynthesis were analyzed by exposing the algal cells to 25 mg/L of SA and JA for 1, 6 and 24 hours, plus to the control (no stress). Based on the RNA-seq analysis, 56,077 unigenes (51.7%) were identified with functions in response to the hormone stress. The top five identified subcategories were cell, cellular process, intracellular, catalytic activity and cytoplasm, which possessed 5600 (~9.99%), 5302 (~9.45%), 5242 (~9.35%), 4407 (~7.86%) and 4195 (~7.48%) unigenes, respectively. Furthermore, 59 unigenes were identified and assigned to 26 putative transcription factors (TFs), including 12 plant-specific TFs. They were likely associated with astaxanthin biosynthesis in Haematococcus upon SA and JA stress. In comparison, the up-regulation of differential expressed genes occurred much earlier, with higher transcript levels in the JA treatment (about 6 h later) than in the SA treatment (beyond 24 h). These results provide valuable information for directing metabolic engineering efforts to improve astaxanthin biosynthesis in H. pluvialis.

Item ID: 41811
Item Type: Article (Research - C1)
ISSN: 1932-6203
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© 2015 Gao et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited

Funders: National Science Foundation of China (NSFC), Hi-Tech Research and Development Program China (HTRDPC), National Natural Science Foundation of Shandong Province (NNSFSP), James Cook Univesity (JCU), Shandong University of Technology (SUT)
Projects and Grants: NSFC 41106124, NSFC 31170279 , HTRDPC 2014AA022003, NNSFSP ZR2011DM006, NNSFSP ZR2011CQ010, JCU 20732, SUT 4072–110045, SUT 4072–114021
Date Deposited: 08 Dec 2015 17:59
FoR Codes: 06 BIOLOGICAL SCIENCES > 0604 Genetics > 060499 Genetics not elsewhere classified @ 50%
06 BIOLOGICAL SCIENCES > 0607 Plant Biology > 060799 Plant Biology not elsewhere classified @ 50%
SEO Codes: 97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 100%
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