Microsatellite multiplex assay for the coral-eating crown-of-thorns starfish, Acanthaster cf. planci

Harrison, Hugo B., Saenz-Agudelo, Pablo, Al-Salamah, Manalle, Messmer, Vanessa, Pratchett, Morgan S., and Berumen, Michael L. (2015) Microsatellite multiplex assay for the coral-eating crown-of-thorns starfish, Acanthaster cf. planci. Conservation Genetics Resources, 7 (3). pp. 627-630.

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Population outbreaks of crown-of-thorns starfish (Acanthaster spp.) represent one of the most significant biological disturbances on Indo-Pacific coral reefs. Here, we combine 15 published and 11 newly isolated polymorphic microsatellite markers from the coral-eating starfish, A. cf. planci and describe their integration into four multiplex PCRs. All markers were polymorphic with a mean of 11.7 ± 1.9 SE alleles per locus and an average observed heterozygosity of 0.619 ± 0.049 SE across 195 genotyped individuals from the Great Barrier Reef. This multiplex assay provides an effective means of investigating the population dynamics of crown-of-thorns starfish and the initiation and spread of population outbreaks.

Item ID: 40895
Item Type: Article (Research - C1)
ISSN: 1877-7260
Keywords: Acanthasteridae; COTS; population outbreak; Great Barrier Reef
Funders: Commonwealth Department of Environment (Reef Plan) , ARC Centre of Excellence for Coral Reef Studies, James Cook University (JCU), King Abdullah University of Science and Technology (KAUST)
Projects and Grants: KAUST URF/1/1389-01-0
Date Deposited: 29 Apr 2016 00:07
FoR Codes: 06 BIOLOGICAL SCIENCES > 0604 Genetics > 060411 Population, Ecological and Evolutionary Genetics @ 50%
06 BIOLOGICAL SCIENCES > 0602 Ecology > 060205 Marine and Estuarine Ecology (incl Marine Ichthyology) @ 50%
SEO Codes: 97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 50%
96 ENVIRONMENT > 9608 Flora, Fauna and Biodiversity > 960808 Marine Flora, Fauna and Biodiversity @ 50%
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