Transgenerational marking of embryonic otoliths in marine fishes using barium stable isotopes

Thorrold, Simon R., Jones, Geoffrey P., Planes, Serge, and Hare, Jonathan H. (2006) Transgenerational marking of embryonic otoliths in marine fishes using barium stable isotopes. Canadian Journal of Fisheries and Aquatic Sciences, 63 (6). pp. 1193-1197.

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Abstract

We describe a new technique for transgenerational marking of embryonic otoliths that promises significant advancements in the study of larval dispersal and population connectivity in marine fishes. The approach is based on maternal transmission of 137Ba from spawning females to egg material that is ultimately incorporated into the otoliths of embryos produced by an individual after exposure to the isotope. We injected females of a benthic-spawning clownfish (Amphiprion melanopus) and a pelagic-spawning serranid (Centropristis striata) with enriched 137BaCl2 and then reared the resulting progeny through to settlement. Barium isotope ratios in the cores of larval otoliths were quantified using laser ablation inductively coupled plasma mass spectrometry. Larval otoliths from both species contained unequivocal Ba isotope signatures over a wide range of doses (0.8–23 µg 137Ba·g female–1). Female A. melanopus continued to produce marked larvae over multiple clutches and for at least 90 days after a single injection. The ability to administer different combinations of stable Ba isotopes provides a new means of mass-marking larvae of benthic- and pelagic-spawning fishes from multiple populations over extended spawning periods.

Item ID: 4086
Item Type: Article (Refereed Research - C1)
Keywords: transgenerational marking; embryonic otoliths; larval dispersal; connectivity; marine fish population
ISSN: 1205-7533
Date Deposited: 29 Oct 2009 01:39
FoR Codes: 05 ENVIRONMENTAL SCIENCES > 0502 Environmental Science and Management > 050202 Conservation and Biodiversity @ 20%
06 BIOLOGICAL SCIENCES > 0602 Ecology > 060205 Marine and Estuarine Ecology (incl Marine Ichthyology) @ 60%
06 BIOLOGICAL SCIENCES > 0602 Ecology > 060207 Population Ecology @ 20%
SEO Codes: 96 ENVIRONMENT > 9608 Flora, Fauna and Biodiversity > 960808 Marine Flora, Fauna and Biodiversity @ 51%
96 ENVIRONMENT > 9613 Remnant Vegetation and Protected Conservation Areas > 961303 Protected Conservation Areas in Marine Environments @ 49%
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