Diversity of toxin and non-toxin containing cyanobacterial mats of meltwater ponds on the Antarctic Peninsula: a pyrosequencing approach

Kleinteich, J., Hildebrand, F., Wood, S.A., Cirés, Samuel, Agha, R., Quesada, A., Pearce, D.A., Convey, P., Küpper, F.C., and Dietrich, D.R. (2014) Diversity of toxin and non-toxin containing cyanobacterial mats of meltwater ponds on the Antarctic Peninsula: a pyrosequencing approach. Antarctic Science, 26 (5). pp. 521-532.

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Abstract

Despite their pivotal role as primary producers, there is little information as to the diversity and physiology of cyanobacteria in the meltwater ecosystems of polar regions. Thirty cyanobacterial mats from Adelaide Island, Antarctica were investigated using 16S rRNA gene pyrosequencing and automated ribosomal intergenic spacer analysis, and screened for cyanobacterial toxins using molecular and chemical approaches. A total of 274 operational taxonomic units (OTUs) were detected. The richness ranged between 8 and 33 cyanobacterial OTUs per sample, reflecting a high mat diversity. Leptolyngbya and Phormidium (c. 55% and 37% of the OTUs per mat) were dominant. Cyanobacterial community composition was similar between mats, particularly those obtained from closely adjacent locations. The cyanotoxin microcystin was detected in 26 of 27 mats (10–300 ng g-1 organic mass), while cylindrospermopsin, detected for the first time in Antarctica, was present in 21 of 30 mats (2–156 ng g-1 organic mass). The latter was confirmed via liquid chromatography-mass spectrometry and by the presence of the cyrAB and cyrJ genes. This study demonstrates the usefulness of pyrosequencing for characterizing diverse cyanobacterial communities, and confirms that cyanobacteria from extreme environments produce a similar range of cyanotoxins as their temperate counterparts.

Item ID: 38773
Item Type: Article (Research - C1)
ISSN: 1365-2079
Keywords: automated ribosomal intergenic spacer analysis; cylindrospermopsin; liquid chromatography-mass spectrometry; microcystin
Funders: UK Natural Environment Research Council (NERC), British Antarctic Survey (BAS), Antarctic Science Bursary, Marie Curie International Research Staff Exchange Scheme Fellowship, Research Foundation Flanders (FWO)
Projects and Grants: Grant AFI-CGS-70
Date Deposited: 14 May 2015 05:15
FoR Codes: 06 BIOLOGICAL SCIENCES > 0602 Ecology > 060202 Community Ecology (excl Invasive Species Ecology) @ 33%
06 BIOLOGICAL SCIENCES > 0603 Evolutionary Biology > 060302 Biogeography and Phylogeography @ 34%
06 BIOLOGICAL SCIENCES > 0605 Microbiology > 060504 Microbial Ecology @ 33%
SEO Codes: 96 ENVIRONMENT > 9603 Climate and Climate Change > 960305 Ecosystem Adaptation to Climate Change @ 33%
96 ENVIRONMENT > 9608 Flora, Fauna and Biodiversity > 960801 Antarctic and Sub-Antarctic Flora, Fauna and Biodiversity @ 34%
97 EXPANDING KNOWLEDGE > 970105 Expanding Knowledge in the Environmental Sciences @ 33%
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