Distribution of the leptospiral immunoglobulin-like (lig) genes in pathogenic Leptospira species and application of ligB to typing leptospiral isolates

Cerqueira, Gustavo M., McBride, Alan J.A., Picardeau, Mathieu, Ribeiro, Samuel G., Moreira, Ângela N., Morel, Viviane, Reis, Mitermayer G., Ko, Albert I., and Dellagostin, Odir A. (2009) Distribution of the leptospiral immunoglobulin-like (lig) genes in pathogenic Leptospira species and application of ligB to typing leptospiral isolates. Journal of Medical Microbiology, 58. pp. 1173-1181.

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DOI: 10.1099/jmm.0.009175-0

View at Publisher Website: http://dx.doi.org/10.1099/jmm.0.009175-0

Abstract

The family of leptospiral immunoglobulin-like (lig) genes comprises ligA, ligB and ligC. This study used PCR to demonstrate the presence of lig genes among serovars from a collection of leptospiral strains and clinical isolates. Whilst ligA and ligC appeared to be present in a limited number of pathogenic serovars, the ligB gene was distributed ubiquitously among all pathogenic strains. None of the lig genes were detected among intermediate or saprophytic Leptospira species. It was also shown that, similar to the previously characterized secY gene, a short specific PCR fragment of ligB could be used to correctly identify pathogenic Leptospira species. These findings demonstrate that ligB is widely present among pathogenic strains and may be useful for their reliable identification and classification.


Item ID:	37424
Item Type:	Article (Research - C1)
ISSN:	1473-5644
Funders:	Brazilian National Research Foundation (BNRF), Oswaldo Cruz Foundation (OCF), National Institute of Health (NIH), Institut Pasteur
Projects and Grants:	OCF 09224-7, OCF PDTIS RVR05, BNRF grant 1.06.0298.00 3773/2005, BNRF grant 420067/2005, BNRF grant 554788/2006, BNRF grant , 473006/2006-5, NIH 5R01 AI0524732D43 TW00919, NIH 2D43 TW00919
Date Deposited:	26 Feb 2015 03:29
FoR Codes:	06 BIOLOGICAL SCIENCES > 0605 Microbiology > 060502 Infectious Agents @ 30% 06 BIOLOGICAL SCIENCES > 0605 Microbiology > 060503 Microbial Genetics @ 30% 06 BIOLOGICAL SCIENCES > 0601 Biochemistry and Cell Biology > 060102 Bioinformatics @ 40%
SEO Codes:	97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 100%
Downloads:	Total: 2
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