Cerqueira, Gustavo M., McBride, Alan J.A., Queiroz, Adriano, Pinto, Luciano S., Silva, Éverton F., Hartskeerl, Rudy A., Reis, Mitermayer G., Ko, Albert I., and Dellagostin, Odir A. (2010) Monitoring Leptospira strain collections: the need for quality control. American Journal of Tropical Medicine and Hygiene, 82 (1). pp. 83-87.

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Abstract

The purpose of this study was to perform a 16S sequence-based quality control of two Leptospira strain collections. 16S rRNA gene sequencing was used to verify two Leptospira reference collections provided by the World Health Organization and maintained at a reference laboratory for leptospirosis in Brazil. Among the 89 serovars evaluated, four conflicting strains were identified in one of the collections. Although 16S rRNA gene sequencing cannot identify Leptospira beyond the species level, it is suitable for the identification of contamination and quality control of leptospiral reference collections. This study highlights the importance of the availability of high-quality 16S rRNA sequences in public databases. In addition, it emphasizes the need for periodical verifications and quality control of Leptospira reference collections.

Item ID:	37422
Item Type:	Article (Research - C1)
ISSN:	1476-1645
Funders:	BioManguinhos, Fiocruz (BM), Brazilian National Research Foundation (BNRF), Research Support Foundation for the State of Bahia, National Institutes of Health (NIH)
Projects and Grants:	BM 09224-7 , BM DTIS RVR05, BNRF grant 1.06.0298.00 3773/2005,, BNRF grant 420067/2005, BNRF grant 554788/2006, NIH R01 AI052473, NIH 2D43 TW00919
Date Deposited:	26 Feb 2015 03:38
FoR Codes:	06 BIOLOGICAL SCIENCES > 0605 Microbiology > 060502 Infectious Agents @ 30% 06 BIOLOGICAL SCIENCES > 0605 Microbiology > 060503 Microbial Genetics @ 30% 06 BIOLOGICAL SCIENCES > 0601 Biochemistry and Cell Biology > 060102 Bioinformatics @ 40%
SEO Codes:	97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 100%
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