Inactivation of clpB in the pathogen Leptospira interrogans reduces virulence and resistance to stress conditions

Lourdault, Kristel, Cerqueira, Gustavo M., Wunder, Elsio A., and Picardeau, Mathieu (2011) Inactivation of clpB in the pathogen Leptospira interrogans reduces virulence and resistance to stress conditions. Infection and Immunity, 79 (9). pp. 3711-3717.

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Abstract

Leptospira interrogans is the causative agent of leptospirosis, which is an emerging zoonotic disease. Resistance to stress conditions is largely uncharacterized for this bacterium. We therefore decided to analyze a clpB mutant that we obtained by random transposon mutagenesis. The mutant did not produce any of the two isoforms of ClpB. The clpB mutant exhibited growth defects at 30° and 37°C and in poor nutrient medium and showed increased susceptibility to oxidative stress, whereas the genetically complemented strain was restored in ClpB expression and in vitro wild-type growth. We also showed that the clpB mutant was attenuated in virulence in an animal model of acute leptospirosis. Our findings demonstrate that ClpB is involved in the general stress response. The chaperone is also necessary, either directly or indirectly, for the virulence of the pathogen L. interrogans.

Item ID: 37413
Item Type: Article (Research - C1)
ISSN: 1098-5522
Funders: Institut Pasteur, National Institute of Health (NIH), French Ministry of Research (FMR), Region Ile-de-France
Projects and Grants: NIH R01 AI052473, NIH U01 AI0088752, NIH D43 TW000919, FMR ANR-08-MIE-018
Date Deposited: 26 Feb 2015 03:20
FoR Codes: 06 BIOLOGICAL SCIENCES > 0605 Microbiology > 060503 Microbial Genetics @ 50%
06 BIOLOGICAL SCIENCES > 0605 Microbiology > 060502 Infectious Agents @ 50%
SEO Codes: 97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 100%
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