Chromosome numbers and DNA content in Bromeliaceae: additional data and critical review

Gitai, Jailson, Paule, Juraj, Zizka, Georg, Schulte, Katharina, and Benko-Iseppon, Ana Maria (2014) Chromosome numbers and DNA content in Bromeliaceae: additional data and critical review. Botanical Journal of the Linnean Society, 176 (3). pp. 349-368.

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Abstract

For the large Neotropical plant family Bromeliaceae, we provide new data on chromosome numbers, cytological features and genome size estimations, and combine them with data available in the literature. Root-tip chromosome counts for 46 species representing four subfamilies and a literature review of previously published data were carried out. Propidium iodide staining and flow cytometry were used to estimate absolute genome sizes in five subfamilies of Bromeliaceae, sampling 28 species. Most species were diploid with 2n = 50 in Bromelioideae, Puyoideae and Pitcairnioideae, followed by 2n = 48 observed mainly in Tillandsioideae. Individual chromosome sizes varied more than tenfold, with the largest chromosomes observed in Tillandsioideae and the smallest in Bromelioideae. Genome sizes (2C-values) varied from 0.85 to 2.23 pg, with the largest genomes in Tillandsioideae. Genome evolution in Bromeliaceae relies on two main mechanisms: polyploidy and dysploidy. With the exception of Tillandsioideae, polyploidy is positively correlated with genome size. Dysploidy is suggested as the mechanism responsible for the generation of the derived chromosome numbers, such as 2n = 32/34 or 2n = 48. The occurrence of B chromosomes in the dysploid genus Cryptanthus suggests ongoing speciation processes closely associated with chromosome rearrangements.

Item ID: 36714
Item Type: Article (Research - C1)
ISSN: 1095-8339
Keywords: B chromosomes, bimodality, bromeliads, C-value, dysploidy, genome size, polyploidy
Funders: Conselho Nacional de Desenvolvimento Científico e Tecnológico, Brazil (CNPq), German Academic Exchange Service (DAAD), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Brazil (CAPES), United Nations Educational, Scientific and Cultural Organization (UNESCO), Fellowship for Molecular Biology and the Deutsche Forschungsgemeinschaft
Projects and Grants: DFG (Zi557/6-2)
Date Deposited: 03 Dec 2014 07:42
FoR Codes: 06 BIOLOGICAL SCIENCES > 0604 Genetics > 060407 Genome Structure and Regulation @ 40%
06 BIOLOGICAL SCIENCES > 0603 Evolutionary Biology > 060310 Plant Systematics and Taxonomy @ 40%
06 BIOLOGICAL SCIENCES > 0603 Evolutionary Biology > 060309 Phylogeny and Comparative Analysis @ 20%
SEO Codes: 97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 100%
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