Microsatellite conservation and Bayesian individual assignment in four Anguilla species
Maes, G.E., Pujolar, J.M., Raeymaekers, J.A.M., Dannewitz, J., and Volckaert, F.A.M. (2006) Microsatellite conservation and Bayesian individual assignment in four Anguilla species. Marine Ecology Progress Series, 319. pp. 251-261.
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Abstract
Microsatellite flanking regions are often highly conserved in fish taxa, enabling their application in other species within or outside the source family. Moderately variable microsatellite markers may also be good candidates for species identification using multi-locus genotypes. We evaluated the degree of conservation of microsatellite flanking regions and the level of polymorphism in 4 commercially important eel species (Anguilla anguilla, A. rostrata, A. japonica and A, marmorata). Using multiplex polymerase chain reactions developed for the first 2 taxa, we assessed the discrimination power of an individual-based assignment method to differentiate all 4 species without initial species information. Detection and classification of each species was performed with high confidence (> 90%), as was assignment of randomly sampled individuals to pre-defined species (> 95%). Our results demonstrate the highly conserved nature of microsatellites and their level of polymorphism in Anguilla species. Although an inverse relationship was found between genetic diversity and differentiation estimates, likely due to homoplasy, assignment proved to be superior to multivariate and distance-based approaches for identifying the 4 species. The method enables the rapid screening of morphologically similar eel species using only 4 co-dominant nuclear loci and the detection of natural hybridisation or anthropogenic mixing between internationally highly traded species.
Item ID: | 34232 |
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Item Type: | Article (Research - C1) |
ISSN: | 1616-1599 |
Keywords: | admixture, anguillids, flanking region, genetic variability, homoplasy, multiplex-PCR, species identification |
Funders: | Belgian Technical Cooperation (BTC), Katholieke Universiteit Leuven (KU Leuven), International Foundation for Science, Mekong River Commission (MRC), Institute for the Promotion of Innovation by Science and Technology in Flanders, Cambodian National and Provincial Departments of Fisheries, Vietnamese Research Institute for Aquaculture |
Projects and Grants: | MRC DANIDA/DoF–Fisher |
Date Deposited: | 20 Aug 2014 04:20 |
FoR Codes: | 06 BIOLOGICAL SCIENCES > 0603 Evolutionary Biology > 060309 Phylogeny and Comparative Analysis @ 50% 06 BIOLOGICAL SCIENCES > 0603 Evolutionary Biology > 060301 Animal Systematics and Taxonomy @ 30% 05 ENVIRONMENTAL SCIENCES > 0502 Environmental Science and Management > 050202 Conservation and Biodiversity @ 20% |
SEO Codes: | 83 ANIMAL PRODUCTION AND ANIMAL PRIMARY PRODUCTS > 8399 Other Animal Production and Animal Primary Products > 839902 Fish Product Traceability and Quality Assurance @ 60% 96 ENVIRONMENT > 9608 Flora, Fauna and Biodiversity > 960808 Marine Flora, Fauna and Biodiversity @ 40% |
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