Blood sampling techniques and storage duration: Effects on the presence and magnitude of the red blood cell β-adrenergic response in rainbow trout (Oncorhynchus mykiss)

Caldwell, Susan, Rummer, Jodie L., and Brauner, Colin J. (2006) Blood sampling techniques and storage duration: Effects on the presence and magnitude of the red blood cell β-adrenergic response in rainbow trout (Oncorhynchus mykiss). Comparative Biochemistry and Physiology-Part A: molecular & integrative physiology, 144 (2). pp. 188-195.

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Abstract

Many teleostean fish, including rainbow trout, regulate red blood cell (RBC) pH (pHi) in the presence of a stress-induced acidosis such as hypoxia, hypercapnia, or exhaustive exercise. This is accomplished through activation of RBC Na⁺/H⁺ exchange (β-NHE), ultimately minimizing impairment to oxygen transport. Presence and characterization of the RBC β-NHE in fish is best tested in blood from cannulated, resting animals; however, several studies have used blood from stressed animals drawn from the caudal vein and stored prior to use. The effects of sampling procedures and storage on the β-NHE response is not known and is the focus of this study. Whole blood drawn from cannulated, resting rainbow trout was compared with RBCs obtained from the caudal vein rinsed and stored at 4 °C for 0, 6, 24, 48, 96 or 144 h. Isoproterenol (10⁻ ⁵ M), a β-adrenergic agonist, was added to hypoxia/hypercapnia incubated RBCs in vitro. In all treatments, isoproterenol induced a large β-NHE response, and storage duration (≤ 96 h) had a minimal affect, indicating that rinsing and storing is an easy and viable means by which to obtain RBCs and investigate function. Storage for 144 h still resulted in a significant RBC β-NHE response; however, viability of RBCs may be compromised.

Item ID: 33080
Item Type: Article (Refereed Research - C1)
Keywords: β-NHE; catecholamine; rainbow trout; blood storage; β-adrenergic response; caudal puncture; cannulation; red blood cells
ISSN: 1531-4332
Funders: Natural Sciences and Engineering Research Council of Canada (NSERC)
Date Deposited: 26 Aug 2016 04:53
FoR Codes: 06 BIOLOGICAL SCIENCES > 0603 Evolutionary Biology > 060309 Phylogeny and Comparative Analysis @ 50%
06 BIOLOGICAL SCIENCES > 0606 Physiology > 060603 Animal Physiology Systems @ 50%
SEO Codes: 97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 100%
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