The effect of ablation pattern on LA-ICPMS analysis of otolith element composition in hake, Merluccius merluccius

Chang, Mei-Yu, Geffen, Audrey J., Kosler, Jan, Dundas, Siv Hjorth, FishPop Trace Consortium, , and Maes, Gregory E. (2012) The effect of ablation pattern on LA-ICPMS analysis of otolith element composition in hake, Merluccius merluccius. Environmental Biology of Fishes, 95 (4). pp. 509-520.

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Abstract

Laser ablation ICPMS (inductively coupled plasma mass spectrometry) analysis is a powerful tool for studies of fish ecology, based on measurement of the chemical composition of otoliths (ear stones). A key trade-off for this analysis is selecting the size of the ablation area to maximize the resolution for discrete temporal intervals during the life of an individual fish, vs the amount of otolith material required to produce reliable data. Three different widths of ablation lines were used to analyze the otoliths of European hake (Merluccius merluccius). The best temporal resolution was produced by ablation lines of 10 μm width, corresponding to less than 2 weeks in the fish's life, but the data from this configuration were variable and often below the detection limit for many elements. Ablation lines of 20 and 30 μm width produced accurate and precise data corresponding to approximately 20 and 30 days in terms of temporal resolution. When tested on hake otoliths, the measured element concentrations differed significantly between the 20 and 30 μm lines. The 30 μm ablation line resulted in a better multivariate model for discrimination between populations, with higher classification success and higher probability of individual assignment to source location.

Item ID: 30017
Item Type: Article (Research - C1)
ISSN: 1573-5133
Keywords: fish otoliths, temporal resolution, laser ablation inductively coupled mass spectrometry, microchemistry, stock discrimination
Funders: European Community’s Seventh Framework Programme, Fund for Scientific Research-Flanders, CalMarO (Marie Curie Initial Training Network)
Projects and Grants: FishPopTrace project KBBE-212399
Date Deposited: 23 Oct 2013 10:27
FoR Codes: 06 BIOLOGICAL SCIENCES > 0602 Ecology > 060205 Marine and Estuarine Ecology (incl Marine Ichthyology) @ 50%
06 BIOLOGICAL SCIENCES > 0608 Zoology > 060809 Vertebrate Biology @ 50%
SEO Codes: 83 ANIMAL PRODUCTION AND ANIMAL PRIMARY PRODUCTS > 8302 Fisheries - Wild Caught > 830204 Wild Caught Fin Fish (excl. Tuna) @ 40%
96 ENVIRONMENT > 9606 Environmental and Natural Resource Evaluation > 960699 Environmental and Natural Resource Evaluation not elsewhere classified @ 30%
96 ENVIRONMENT > 9605 Ecosystem Assessment and Management > 960507 Ecosystem Assessment and Management of Marine Environments @ 30%
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