The combined effects of temperature and CO₂ lead to altered gene expression in Acropora aspera

Ogawa, D., Bobeszko, T., Ainsworth, T., and Leggat, W. (2013) The combined effects of temperature and CO₂ lead to altered gene expression in Acropora aspera. Coral Reefs, 32 (4). pp. 895-907.

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Abstract

This study explored the interactive effects of near-term CO₂ increases (40–90 ppm above current ambient) during a simulated bleaching event (34°C for 5d) of Acropora aspera by linking physiology to expression patterns of genes involved in carbon metabolism. Symbiodinium photosynthetic efficiency (F(v)/F(m)) was significantly depressed by the bleaching event, while elevated pressure of CO₂ (pCO₂) slightly mitigated the effects of increased temperature on F(v)/F(m) during the final 4 d of the recovery period, however, did not affect the loss of symbionts. Elevated pCO₂ alone had no effect on F(v)/F(m) or symbiont density. Expression of targeted Symbiodinium genes involved in carbon metabolism and heat stress response was not significantly altered by either increased temperature and/or CO₂. Of the selected host genes, two carbonic anhydrase isoforms (coCA2 and coCA3) exhibited the largest changes, most notably in crossed bleaching and elevated pCO₂ treatments. CA2 was significantly down-regulated on day 14 in all treatments, with the greatest decrease in the crossed treatment (relative expression compared to control = 0.16; p < 0.05); CA3 showed a similar trend, with expression levels 0.20-fold of controls on day 14 (p<0.05) in the elevated temperature/pCO₂ treatment. The synergistic effects of ocean acidification and bleaching were evident during this study and demonstrate that increased pCO₂ in surface waters will impact corals much sooner than many studies utilising end-of-century pCO₂ concentrations would indicate.

Item ID: 27887
Item Type: Article (Research - C1)
ISSN: 1432-0975
Keywords: coral, acidification, bleaching, gene expression
Funders: Australian Research Council (ARC)
Date Deposited: 02 Jul 2013 23:45
FoR Codes: 06 BIOLOGICAL SCIENCES > 0604 Genetics > 060405 Gene Expression (incl Microarray and other genome-wide approaches) @ 60%
06 BIOLOGICAL SCIENCES > 0607 Plant Biology > 060702 Plant Cell and Molecular Biology @ 40%
SEO Codes: 96 ENVIRONMENT > 9603 Climate and Climate Change > 960305 Ecosystem Adaptation to Climate Change @ 80%
96 ENVIRONMENT > 9608 Flora, Fauna and Biodiversity > 960808 Marine Flora, Fauna and Biodiversity @ 20%
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