Isolation and characterization of expressed sequence tag-linked microsatellite loci for the European eel (Anguilla anguilla)

Pujolar, J.M., Maes, G.E., Van Houdt, J.K.J., and Zane, L. (2009) Isolation and characterization of expressed sequence tag-linked microsatellite loci for the European eel (Anguilla anguilla). Molecular Ecology Resources, 9 (1). pp. 233-235.

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Abstract

A European eel (Anguilla anguilla) expressed sequence tag database consisting of 795 contigs and 4008 singletons was screened for microsatellites sequences. Primers were designed to amplify 96 repeats, of which 86 gave good quality amplification products. Twenty-eight microsatellites were selected for further microsatellite genotyping. Only two loci were found to be monomorphic; out of the 26 polymorphic loci, number of alleles per locus ranged from two to 14, while the observed and expected heterozygosities ranged from 0.05 to 0.93, and from 0.05 to 0.95, respectively. All 28 primer sets tested revealed positive amplification in American eel (Anguilla rostrata).

Item ID: 27626
Item Type: Article (Short Note)
ISSN: 1755-0998
Keywords: Anguilla, EST, European eel, microsatellite
Date Deposited: 07 Jun 2013 09:37
FoR Codes: 06 BIOLOGICAL SCIENCES > 0601 Biochemistry and Cell Biology > 060102 Bioinformatics @ 40%
06 BIOLOGICAL SCIENCES > 0604 Genetics > 060405 Gene Expression (incl Microarray and other genome-wide approaches) @ 30%
06 BIOLOGICAL SCIENCES > 0604 Genetics > 060411 Population, Ecological and Evolutionary Genetics @ 30%
SEO Codes: 83 ANIMAL PRODUCTION AND ANIMAL PRIMARY PRODUCTS > 8302 Fisheries - Wild Caught > 830204 Wild Caught Fin Fish (excl. Tuna) @ 50%
96 ENVIRONMENT > 9608 Flora, Fauna and Biodiversity > 960808 Marine Flora, Fauna and Biodiversity @ 50%
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